In the present study, a statistical tool called the simplex lattice mixture design method was used to create a new formulation of Natural Deep Eutectic Solvent (NADES), which is derived from a combination of three compounds (citric acid, glycerol, and water) to extract bioactive compounds from chickpea (Cicer arietinum L.) sprouts. The mixture (natural deep eutectic solvent) was formulated by combining three solvents including citric acid, glycerol, and water. The extraction was performed in a sonication bath for 30 min. The simultaneous optimization was performed to obtain the highest total polyphenol content (TPC), total flavonoid content (TFC) and antioxidants activity. The highest values of total polyphenol content (TPC), total flavonoid content (TFC) and antioxidant activity were 128.0 ± 0.2 mg GAE/100 g, 38.61 ± 0.03 mg CE/100 g and 2117 ± 1.8 µmol TE/100 g respectively. HPLC-DAD of the optimized extract was utilized for quantification of polyphenol compounds showing catechin as the main compound followed by chlorogenic acid, epicatechin, syringic acid, rutin, gallic acid, kaempferol 3-glucoside, ferulic acid, and coumaric acid. These findings may represent a significant advancement in the management of phenolic compound extraction for targeted uses, such as serving as alternatives to traditional antioxidants primarily employed in the food industry to improve nutritional quality. Furthermore, our research has shown that mixture designs are an efficient and useful method for structuring and optimizing experimental parameters to achieve the most accurate results with the minimum number of experiments.

中文翻译：

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使用单纯形晶格混合物设计方法优化鹰嘴豆 （Cicer arietinum L.） 芽中生物活性化合物的 UAE-NADES 绿色萃取


在本研究中，使用一种称为单纯形晶格混合物设计方法的统计工具来创建天然深共晶溶剂 （NADES） 的新配方，该配方源自三种化合物（柠檬酸、甘油和水）的组合，用于从鹰嘴豆 （Cicer arietinum L.） 芽中提取生物活性化合物。混合物（天然低共熔溶剂）是通过混合三种溶剂配制的，包括柠檬酸、甘油和水。萃取在超声处理槽中进行 30 分钟。同时进行优化以获得最高的总多酚含量 （TPC） 、总类黄酮含量 （TFC） 和抗氧化活性。总多酚含量 （TPC）、总黄酮含量 （TFC） 和抗氧化活性最高值分别为 128.0 ± 0.2 mg GAE/100 g、38.61 ± 0.03 mg CE/100 g 和 2117 ± 1.8 μmol TE/100 g。利用优化提取物的 HPLC-DAD 定量显示儿茶素为主要化合物的多酚化合物，其次是绿原酸、表儿茶素、丁香酸、芦丁、没食子酸、山奈酚 3-葡萄糖苷、阿魏酸和香豆酸。这些发现可能代表了针对特定用途的酚类化合物提取管理的重大进展，例如作为主要用于食品工业的传统抗氧化剂的替代品，以提高营养质量。此外，我们的研究表明，混合物设计是一种构建和优化实验参数的有效方法，以最少的实验次数获得最准确的结果。