A bioactive film was developed using cricket (Acheta domestica) protein (Cric-Prot) and Caralluma fimbriata extract-based nanoparticles [Car-Fim-NPs (0.0, 1.0, 2.0, and 3.0 % w/v)] to augment the storage stability and functional value of cheddar cheese (Ched-Chee). The Car-Fim-NPs were developed using an ultrasonication-mediated (20 kHz, 500 W, 10 min, pulse duration of 5/5 s on/off) green method. The addition of Car-Fim-NPs modified the film characteristics [density (%), WVTR (mg/mt2), transmittance (%), elongation at break (%), and colour (L*, a*, b*)] and enhanced its antioxidant [DPPH, ABTS and FRAP activities, total phenolic and flavonoid contents, and antioxidant release (%)] and antimicrobial (inhibitory halos against E. coli and S. aureus) potential. Application of the films containing Car-Fim-NPs (1.0–3.0 %) increased the antioxidant potential (DPPH, ABTS and FRAP activities), lipid (TBARS and free fatty acids) and protein (total carbonyl content) oxidative stability, and microbial quality (microbial counts) of the Ched-Chee during 90 days of storage. The sensory quality of Ched-Chee showed a significant increase after day 30 till the storage end. Gastrointestinal digestion simulation augmented the antioxidant potential of Ched-Chee. Overall, the results indicate the possibility of theuse ofCric-Prot-based film containing Car-Fim-NPs to enhance thestorage quality of Ched-Chee.

中文翻译：

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含有基于 Caralluma fimbriata 提取物的纳米颗粒的基于蟋蟀蛋白的薄膜，用于保存切达干酪


使用蟋蟀 （Acheta domestica） 蛋白 （Cric-Prot） 和基于 Caralluma fimbriata 提取物的纳米颗粒 [Car-Fim-NPs （0.0、1.0、2.0 和 3.0 % w/v）] 开发了一种生物活性膜，以提高切达干酪 （Ched-Chee） 的储存稳定性和功能价值。Car-Fim-NPs 是使用超声介导的 （20 kHz， 500 W， 10 min， 脉冲持续时间 5/5 s 开/关） 绿色方法开发的。Car-Fim-NPs 的添加改变了薄膜特性 [密度 （%）、WVTR （mg/mt2）、透射率 （%）、断裂伸长率 （%）和颜色 （L*、a*、b*）]，并增强了其抗氧化 [DPPH、ABTS 和 FRAP 活性、总酚类和类黄酮含量以及抗氧化释放 （%）] 和抗菌（针对大肠杆菌和金黄色葡萄球菌的抑制光晕）潜力。在储存 90 天期间，含有 Car-Fim-NPs （1.0–3.0%） 的薄膜增加了切果的抗氧化潜力（DPPH、ABTS 和 FRAP 活性）、脂质（TBARS 和游离脂肪酸）和蛋白质（总羰基含量）、氧化稳定性以及微生物质量（微生物计数）。Ched-Chee 的感官品质在 30 d 后至贮藏结束期间表现出显著增加。胃肠道消化模拟增强了 Ched-Chee 的抗氧化潜力。总体而言，结果表明使用含有 Car-Fim-NPs 的基于 Cric-Prot 的薄膜来提高 Ched-Chee 的储存质量的可能性。