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Matrix glycosaminoglycans and proteoglycans in human cornea organoids and similarities with fetal corneal stages
The Ocular Surface ( IF 5.9 ) Pub Date : 2024-11-28 , DOI: 10.1016/j.jtos.2024.11.007
Sean Ashworth, Manas Dhanuka, Alireza Khodadadi-Jamayran, Madhuri Amulya Koduri, George Maiti, Shukti Chakravarti

We developed human cornea organoids (HCOs) from induced pluripotent stem cells (iPSCs) where single-cell RNA-sequence (scRNA-seq) analysis suggested similarity with developing rather than mature human corneas. We performed immunohistology to determine the presence of corneal glycosaminoglycans as an assessment of maturity.

中文翻译:


人角膜类器官中的基质糖胺聚糖和蛋白聚糖及其与胎儿角膜分期的相似性



我们从诱导多能干细胞 (iPSC) 开发了人角膜类器官 (HCO),其中单细胞 RNA 序列 (scRNA-seq) 分析表明与发育中的而不是成熟的人角膜相似。我们进行了免疫组织学检查以确定角膜糖胺聚糖的存在,作为成熟度的评估。
更新日期:2024-11-28
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