DJ-1 is a protein whose mutation causes rare heritable forms of Parkinson's disease (PD) and is of interest as a target for treating PD and other disorders. This work used high performance affinity microcolumns to screen and examine the binding of small molecules to DJ-1, as could be used to develop new therapeutics or to study the role of DJ-1 in PD. Non-covalent entrapment was used to place microgram quantities of DJ-1 in an unmodified form within microcolumns, which were then used in multiple studies to analyze binding by model compounds and possible drug candidates to DJ-1.

中文翻译：

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开发和使用 DJ-1 亲和微柱筛选和研究帕金森病的小候选药物


DJ-1 是一种蛋白质，其突变会导致罕见的遗传性帕金森病 （PD），并作为治疗 PD 和其他疾病的靶标而受到关注。这项工作使用高性能亲和微柱来筛选和检查小分子与 DJ-1 的结合，可用于开发新的治疗方法或研究 DJ-1 在 PD 中的作用。非共价包埋用于将微克级的 DJ-1 以未修饰的形式放置在微柱中，然后将其用于多项研究，以分析模型化合物和可能的候选药物与 DJ-1 的结合。