Lipid droplets and lysosomes are crucial organelles that play significant roles in cellular metabolism. Therefore, studying both lipid droplets and lysosomes simultaneously holds great value. A new fluorescent probe BTD-Lyso was designed based on the principle of intramolecular charge transfer (ICT). The probe was formed with a typical D-π-A structure, and it used benzothiadiazole as the electronic acceptor and morpholine as the electronic donor. The experimental results showed that BTD-Lyso was able to detect the change of polarity in the microenvironment. Furthermore, the probe showed a large Stokes shift (300 nm), excellent selectivity, polarity sensitivity, and photostability. In addition, it could be successfully utilized in cellular imaging due to its low cytotoxicity and could target lipid droplets and lysosomes well.

中文翻译：

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一种靶向溶酶体和脂滴的具有大 Stokes 位移的新型荧光探针及其在细胞成像中的应用


脂滴和溶酶体是在细胞代谢中起重要作用的重要细胞器。因此，同时研究脂滴和溶酶体具有很大的价值。基于分子内电荷转移 （ICT） 原理设计了一种新的荧光探针 BTD-Lyso。探针采用典型的 D-π-A 结构形成，以苯并噻二唑为电子受体，吗啉为电子供体。实验结果表明，BTD-Lyso 能够检测到微环境中极性的变化。此外，探针显示出较大的斯托克斯位移 （300 nm）、优异的选择性、极性敏感性和光稳定性。此外，由于其低细胞毒性，它可以成功用于细胞成像，并且可以很好地靶向脂滴和溶酶体。