Carbon monoxide (CO) is a colorless, odorless, toxic, and polluting gas produced by the incomplete combustion of carbon-containing substances such as coal and petroleum. CO is also a gaseous signaling molecule, which participates in and regulates a series of physiological and pathological processes. In this work, two kinds of CO-activated fluorescence probes 2 and 4 were designed and synthesized for the rapid detection of CO and evaluation of acute CO poisoning by hyperbaric oxygen therapy. Specifically, 2 and 4 both used the allyl formate group as the CO response unit and when they reacted with CO, the response group broke off and the fluorescence burst effect subsided, allowing them to regain fluorescence. In addition, because of the excellent optical properties of 4 (especially, colorimetric and near-infrared (NIR) fluorescence), subsequent experiments were performed with 4 as a representative for imaging CO in zebrafish and mice with impressive results. Interestingly, 4 has been successfully used for assessing acute CO poisoning by hyperbaric oxygen therapy with satisfactory results, revealing practical application in the connection between CO and hyperbaric oxygen therapy.

中文翻译：

---

构建用于 CO 检测的稳健荧光探针及评估高压氧治疗急性 CO 中毒的疗效


一氧化碳 （CO） 是一种无色、无味、有毒和污染性的气体，由煤和石油等含碳物质不完全燃烧产生。CO 也是一种气态信号分子，参与并调节一系列生理和病理过程。在这项工作中，设计合成了两种 CO 激活荧光探针 2 和 4，用于快速检测 CO 和评估高压氧治疗急性 CO 中毒。具体来说，2 和 4 都使用甲酸烯丙酯基团作为 CO 响应单元，当它们与 CO 反应时，响应组中断，荧光突发效应消退，使它们重新获得荧光。此外，由于 4 具有出色的光学特性（尤其是比色和近红外 （NIR） 荧光），随后以 4 作为斑马鱼和小鼠 CO 成像的代表进行了实验，并取得了令人印象深刻的结果。有趣的是，4 已成功用于评估高压氧治疗引起的急性 CO 中毒，结果令人满意，揭示了 CO 与高压氧治疗之间联系的实际应用。