The real-time and full concentration analysis of ethanol during the fermentation reaction could reduce product inhibition, thereby promoting productivity. However, only a few techniques can directly detect the fermentation broth without pretreatment. To address this issue, we proposed an ultrasensitive biosensing microchip to realize the precise determination of ethanol concentrations in the original fermentation broth, which relied on the construction of a Prussian blue (PB)/Au nanoflower architecture as the recognition probe. Since the in situ growth of the nanoflowers, a biosensing microchip was functionalized to accurately recognize the ethanol within only 9 s. Using this biosensor to monitor and control the ethanol concentration in the whole 109 h fermentation production, the ethanol yield has been increased from 47.1% to 50.09%, and the average fermentation time has been reduced from 44 to 27.25 h to successfully cut down the product inhibition during the whole industrial fermentation process.

中文翻译：

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超灵敏生物传感微芯片控制乙醇发酵，有效降低产品抑制


发酵反应过程中乙醇的实时和全浓度分析可以减少产物抑制，从而提高生产率。然而，只有少数技术可以在不进行预处理的情况下直接检测发酵液。为了解决这个问题，我们提出了一种超灵敏的生物传感微芯片，以实现对原始发酵液中乙醇浓度的精确测定，它依赖于普鲁士蓝 （PB）/Au 纳米花结构的构建作为识别探针。由于纳米花的原位生长，生物传感微芯片被功能化，可在 9 秒内准确识别乙醇。使用该生物传感器监测和控制整个 109 h 发酵生产中的乙醇浓度，乙醇收率从 47.1% 提高到 50.09%，平均发酵时间从 44 小时减少到 27.25 h，成功降低了整个工业发酵过程中的产品抑制。