Understanding the distribution and abundance of microbial stress-related genes (SRGs) is crucial for unraveling the intricate dynamics of microbial communities and their responses to diverse environmental conditions. Despite the importance of these genes in shaping microbial adaptation and resilience, a comprehensive examination of their relative abundance across distinct climatic regions, such as boreal, temperate, and tropical environments, remains notably scarce in the existing literature. This study seeks to address this research gap by exploring and comparing the relative abundances of microbial SRGs using GeoChip 5.0 functional gene array in these three climatic regions. Our data indicate a greater relative abundance of SRGs such as nsrR, degP, cpxR, ahpF, Obg and cisD in the tropical region than in the other two regions whereas ecf and glnA showed higher abundance in both temperate and tropical regions in terms of reference forests and old parks. The distinct characteristics of the tropical biome are likely to influence both SRGs and the profile of SRG-hosts, consequently enhancing soil microbial abundances. Moreover, multivariate analyses unveiled distinct SRG profiles in urban parks and climatic regions. Additionally, we explored the influence of plant functional types (recalcitrant and labile tree litter, lawn) on SRGs. The impact of vegetation type on SRGs was not statistically significant, except in the tropical region, where its influence was notably pronounced compared to the boreal and temperate regions. Furthermore, several genes (nsrR, degP, cpxR, degS, dnaK, Obg, cisD, and glnA) were more prevalent in old parks when compared to young parks in all three climatic regions. However, urban parks displayed a significantly elevated abundance of SRGs compared to forest areas. This study provides new insights into the prevalence of SRGs across different climatic regions, enhancing our understanding of their dynamics in both human-influenced environments and pristine habitats.

中文翻译：

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城市公园微生物胁迫响应基因的跨气候比较：比较分析


了解微生物应激相关基因 （SRG） 的分布和丰度对于解开微生物群落的复杂动态及其对不同环境条件的反应至关重要。尽管这些基因在塑造微生物适应和恢复力方面很重要，但在现有文献中，对它们在不同气候区域（如北方、温带和热带环境）的相对丰度的全面研究仍然非常稀缺。本研究旨在通过使用 GeoChip 5.0 功能基因阵列探索和比较这三个气候区微生物 SRG 的相对丰度来解决这一研究空白。我们的数据表明，热带地区 nsrR、degP、cpxR、ahpF、Obg 和 cisD 等 SRG 的相对丰度高于其他两个地区，而 ecf 和 glnA 在温带和热带地区在参考林和老公园方面均表现出更高的丰度。热带生物群落的独特特征可能会影响 SRG 和 SRG 宿主的概况，从而提高土壤微生物的丰度。此外，多变量分析揭示了城市公园和气候区域的不同 SRG 概况。此外，我们探讨了植物功能类型 （顽固和不稳定的树木凋落物、草坪） 对 SRG 的影响。植被类型对 SRG 的影响无统计学意义，但热带地区除外，与北方和温带地区相比，其影响明显明显。此外，与所有三个气候区的年轻公园相比，几个基因 （nsrR、degP、cpxR、degS、dnaK、Obg、cisD 和 glnA） 在老公园中更为普遍。 然而，与森林地区相比，城市公园的 SRG 丰度显著升高。这项研究为不同气候区域 SRG 的普遍性提供了新的见解，增强了我们对它们在人类影响环境和原始栖息地中的动态的理解。