Previous studies found that the multi-component mixtures with hormesis concentration-response curves (CRCs) were divided into three types according to the combined toxicity analysis of the segment-based method and σ2(k∙ECx) (the variance of k∙ECx). In this study, the acute and long-term toxicity of six pollutants and 12 six-component mixtures were assessed using microplate toxicity analyses (MTA). The functional relationship between σ2(k·ECx) and effect ratio (ERx) was determined by means of the independent action (IA) and the ER model to systematically investigate the correlation between mixture types in acute and long-term toxicity. The results indicated that across the entire concentration range, the mixture type of acute toxicity was consistent with short time exposure (0.25 h) measured in the long-term toxicity experiment. In the inhibition effect range, the types of mixtures of acute toxicity remained consistent with the chronic toxicity (exposure for 24 h) in 11 of the 12 mixtures. This study clarified the changes in the joint action of multi-component mixtures on Aliivibrio fischeri in terms of acute and long-term toxicity. The chronic toxicity of the mixtures can be predicted from the acute toxicity results, which provides a theoretical basis for the biological toxicity evaluation of multi-component mixtures.

中文翻译：

---

基于浓度响应曲线的六组分混合物在急性和长期毒性测定中的形态学参数的联合作用


以往的研究发现，根据分段法和 σ2（k∙ECx） （k∙ECx 的方差）的组合毒性分析，将具有激效浓度-响应曲线 （CRCs） 的多组分混合物分为 3 种类型。在这项研究中，使用微孔板毒性分析 （MTA） 评估了 6 种污染物和 12 种六组分混合物的急性和长期毒性。σ2（k·ECx） 和效应比 （ERx） 通过独立作用 （IA） 和 ER 模型确定，以系统研究混合物类型在急性和长期毒性中的相关性。结果表明，在整个浓度范围内，急性毒性的混合物类型与长期毒性实验中测得的短时间暴露 （0.25 h） 一致。在抑制效果范围内，12 种混合物中 11 种的急性毒性混合物类型与慢性毒性 （暴露 24 h） 保持一致。本研究阐明了多组分混合物对 Aliivibrio fischeri 的联合作用在急性和长期毒性方面的变化。从急性毒性结果可以预测混合物的慢性毒性，为多组分混合物的生物毒性评价提供了理论依据。