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Corrigendum to: Anthocyanin Fruit encodes an R2R3-MYB transcription factor, SlAN2-like, activating the transcription of SlMYBATV to fine-tune anthocyanin content in tomato fruit
New Phytologist ( IF 8.3 ) Pub Date : 2024-11-28 , DOI: 10.1111/nph.20296


New Phytologist, 225(2020), 2048–2063, doi: 10.1111/nph.16272.

Since its publication, it has been brought to our attention that there are errors in the article by Yan et al. (2023). Some of the images in Figs 7, 8 & S11 were duplicated in error during the compilation of these figures. The correct Figs 7, 8 & S11, and the associated legends, are given below.

We apologize to our readers for these errors.

Corrected Figs 7, 8 & S11:

Details are in the caption following the image
Fig. 7
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Interaction of SlMYBATV or anthocyanin fruit (Aft) with SlAN1 or SlJAF13 by a yeast two-hybrid assay. Three transcripts of SlMYBATV (SlMYBATV-X1 to -X3) identified in a previous study (Cao et al., 2017) were used. SlMYBATV-X2 and -X3 both lead to truncated proteins compared with SlMYBATV-X1. Co-transformants containing pGADT7-RecT with pGBDT7-53 were used as a positive control; co-transformants containing pGADT7-RecT with pGBDT7-Lam were used as a negative control. The assays shown in this figure and Supporting Information Fig. S11 were carried out together; therefore, the same positive and negative controls are used in both figures.
Details are in the caption following the image
Fig. 8
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Interaction of anthocyanin fruit (Aft) with the SlMYBATV promoter in vivo. (a) DNase I hypersensitivity site (DHS) flanking the SlMYBATV gene. The DNase hypersensitivity data are from a previous publication (Qiu et al., 2016a). The data were visualized by the Integrative Genomics Viewer. The red arrow indicates the DHS. (b) Identification of motifs in the DHS and the predicted promoter region (0 bp to _2000 bp from ATG) of SlMYBATV. Motif discovery and ontology analysis were performed using MEME Suite 5.0.5. (c) A yeast one-hybrid assay shows that Aft binds to MYB-enriched fragment (pATV) in the SlMYBATV promoter and its mutants (pATV -M1 to M5, -M1 to -M5 means deletion in the first to fifth MYB motif, respectively). Co-transformants containing different constructs (pGADT7-Aft with pATV, and pATV -M1 to -M5, respectively) were grown on SD/−Leu and SD/−Leu media plus Aureobasidin A (AbA) for 3 d at 30°C. Co-transformants containing pGADT7-53 with p53-AbAi were used as a positive control; co-transformants containing pGADT7 with pATV were used as a negative control. (d) Relative luciferase activity (the ratio of LUC to REN) of pATV by Aft. Data are means of six biological replicates_SE. Asterisks indicate significant difference: **, P < 0.01 (Student's t-test).
Details are in the caption following the image
Fig. S11
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Interaction of SlMYBATV with SlAN1 by a yeast two-hybrid assay. Three transcripts of SlMYBATV (SlMYBATV-X1 to -X3) identified in the previous study (Cao et al., 2017) were used. SlMYBATV-X2 and -X3 both lead to truncated proteins compared with SlMYBATV-X1. Co-transformants containing pGADT7-RecT with pGBDT7-53 were used as a positive control; co-transformants containing pGADT7RecT with pGBDT7-Lam were used as a negative control. The assays shown in this figure and Fig. 7 were carried out together; therefore, the same positive and negative controls are used in both figures.


中文翻译:


勘误到:花青素果实编码 R2R3-MYB 转录因子,SlAN2 样,激活 SlMYBATV 的转录以微调番茄果实中的花青素含量


New Phytologist, 225(2020), 2048–2063, doi: 10.1111/nph.16272.


自发表以来,我们注意到 Yan 等 人的文章中存在错误。(2023). 图7、8和S11中的一些图像在编译这些数字的过程中被错误地复制了。正确的图7、8和S11以及相关的图例如下给出。


对于这些错误,我们向读者道歉。


修正了图 7、8 和 S11:

Details are in the caption following the image
 图 7

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通过酵母双杂交测定 SlMYBATV 或花青素果实 (Aft) 与 SlAN1 或 SlJAF13 的相互作用。使用了在先前研究 (Caoet al., 2017) 中鉴定的 SlMYBATV 的三个转录本 (SlMYBATV-X1 至 -X3)。与 SlMYBATV-X1 相比,SlMYBATV-X2-X3 都会导致截短蛋白。含有 pGADT7-RecT 和 pGBDT7-53 的共转化体用作阳性对照;含有 pGADT7-RecT 和 pGBDT7-Lam 的共转化体用作阴性对照。此图和支持信息图 1 中显示的分析方法。S11 一起进行;因此,两个图中使用相同的阳性和阴性对照。
Details are in the caption following the image
 图 8

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花青素果实 (Aft) 与 SlMYBATV 启动子在体内的相互作用。(a) SlMYBATV 基因侧翼的 DNase I 超敏反应位点 (DHS)。DNase 超敏反应数据来自以前的出版物(Qiu et al., 2016a)。数据通过 Integrative Genomics Viewer 进行可视化。红色箭头表示 DHS。(b) 在 DHS 和 SlMYBATV 的预测启动子区域 (0 bp 至 _2000 bp) 中鉴定基序。使用 MEME Suite 5.0.5 进行基序发现和本体分析。(c) 酵母单杂交测定显示 Aft 与 SlMYBATV 启动子及其突变体中富含 MYB 的片段 (pATV) 结合 (pATV -M1 至 M5,-M1 至 -M5 分别表示第一至第五 MYB 基序中的缺失)。含有不同构建体(分别为 pGADT7-Aft 与 pATV 和 pATV -M1 至 -M5)的共转化体在 SD/-Leu 和 SD/-Leu 培养基加 Aureobasidin A (AbA) 上在 30°C 下生长 3 天。 含有 pGADT7-53 和 p53-AbAi 的共转化体用作阳性对照;含有 pGADT7 和 pATV 的共转化体用作阴性对照。(d) 船尾 pATV 的相对荧光素酶活性(LUC 与 任 的比率)。数据是 6 个生物replicates_SE的平均值。星号表示显著差异: **, P < 0.01 (学生 t 检验)。
Details are in the caption following the image
 无花果。第 11 季

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通过酵母双杂交测定 SlMYBATV 与 SlAN1 的相互作用。使用了在先前研究 (Cao et al., 2017) 中鉴定的 SlMYBATV 的三个转录本 (SlMYBATV-X1-X3)。与 SlMYBATV-X1 相比,SlMYBATV-X2-X3 都会导致截短蛋白。含有 pGADT7-RecT 和 pGBDT7-53 的共转化体用作阳性对照;含有 pGADT7RecT 和 pGBDT7-Lam 的共转化体用作阴性对照。该图和图 7 所示的分析一起进行;因此,两个图中使用相同的阳性和阴性对照。
更新日期:2024-11-28
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