Two pilot-scale tertiary moving bed biofilm reactor (MBBR) treatment trains were operated onsite for 371 days in a local wastewater treatment plant (WWTP) to compare their treatment performance and mechanistic difference when methanol and glycerol were used as carbon sources, respectively. Both trains were able to meet the tertiary effluent total inorganic nitrogen (TIN) requirement of < 3 mg/L, with 31.6% ∼ 46.3 % methanol savings or 30.9 % ∼ 43.8 % glycerol savings over full denitrification projected at influent dissolved oxygen in the range of 0∼3 mg/L. Very different nitrite provision mechanism was found between the two types of carbon sources, i.e., the nitrite sink by anammox through its outcompetition of dentification was the major source of nitrite provision mechanism for anammox bacteria when methanol was used as a carbon; while the rate differential between denitratation and denitritation was the major nitrite source when glycerol was used as a carbon. The cause of this mechanistic discrepancy can be ascribed to the dramatic different half-saturation constants between the two types of carbon sources (e.g., half saturation constant of glycerol was 1.7 times that of methanol). This study provided fundamental understandings that can be used to reconcile the controversy over whether methanol is suitable for partial denitrification anammox in low strength wastewater treatment.

中文翻译：

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对处理实际二次流出物的三级移动床生物膜反应器中甲醇和甘油进料部分反硝化厌氧氨氧化性能差异的机理理解


两套中试规模的三级移动床生物膜反应器 （MBBR） 处理列车在当地污水处理厂 （WWTP） 现场运行 371 天，以分别比较使用甲醇和甘油作为碳源时的处理性能和机理差异。两条生产线都能够满足 < 3 mg/L 的三级流出总无机氮 （TIN） 要求，在 0∼3 mg/L 的进水溶解氧范围内，预计在完全反硝化时节省 31.6% ∼ 46.3 % 的甲醇或 30.9% ∼ 43.8% 的甘油。在两种类型的碳源之间发现了非常不同的亚硝酸盐供应机制， 即，当使用甲醇作为碳时，厌氧氨氧化物质通过其竞争识别而沉入亚硝酸盐是厌氧氨氧化细菌亚硝酸盐供应机制的主要来源;而当甘油用作碳时，反硝化和反硝化之间的速率差异是主要的亚硝酸盐来源。这种机理差异的原因可归因于两种碳源之间的半饱和常数截然不同（例如，甘油的半饱和常数是甲醇的 1.7 倍）。这项研究提供了基本理解，可用于调和关于甲醇是否适合在低强度废水处理中用于部分反硝化厌氧氨氧化的争议。