BACKGROUNDBacterial leaf blight caused by the bacterium Xanthomonas oryzae pv. oryzae has a substantial effect on the yield of rice crops. The secretory (Sec) pathway, essential for efflux transport in bacteria, remains insufficiently studied in X. oryzae pv. oryzae, especially regarding its roles in drug resistance and physiology.RESULTSThis study involved transcriptome analysis on two X. oryzae pv. oryzae strains: a secG deletion strain (∆secG) and its complemented strain (C: ∆secG). In comparison to the parental strain PXO99A, ∆secG exhibited slower growth, with reductions in swimming (20.67%) and swarming (12.59%), while maintaining 76.7% of its biofilm formation capacity and 63.6% of exopolysaccharide production. The minimum inhibitory concentration (MIC50) values for an n‐butanol extract of Bacillus velezensis HN‐2 (HN‐2E) and bacitracin against ∆secG were 0.426 μg/mL (5.3% lower than that of PXO99A) and 10.905 μg/mL, respectively. Notably, ∆secG exhibited increased susceptibility to hydrogen peroxide (H2O2), being inhibited at 0.25 mm compared to 0.3 mm for PXO99A and C: ∆secG. In the presence of 0.2 mM H2O2, the susceptibility of ∆secG to HN‐2E increased by 31.22% (MIC50 = 0.159 μg/mL), while PXO99A and C: ∆secG exhibited MIC50 values of 0.280 and 0.291 μg/mL, respectively.CONCLUSIONOur findings demonstrate that Bacillus‐induced H2O2 production enhances the sensitivity of X. oryzae pv. oryzae to biocontrol agents, providing valuable insights for the prevention of bacterial leaf blight. These results highlight the significance of the Sec pathway in the behavior and resistance of X. oryzae pv. oryzae, as well as potential areas for further research on plant diseases. © 2024 Society of Chemical Industry.

中文翻译：

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secG 基因突变对米黄单胞菌 pv 耐药性和生理生化活性的影响。水稻


背景由细菌 Xanthomonas oryzae pv 引起的疫叶枯病。米序对水稻作物的产量有很大影响。分泌 （Sec） 途径对细菌外排运输至关重要，但在 X. oryzae pv 中仍未得到充分研究。水稻，特别是关于它在耐药性和生理学中的作用。结果该研究涉及对两个米 X. oryzae pv.米质菌株：secG 缺失菌株 （∆secG） 及其互补菌株 （C： ∆secG）。与亲本菌株 PXO99A 相比，∆secG 生长较慢，游泳 （20.67%） 和蜂群 （12.59%） 减少，同时保持 76.7% 的生物膜形成能力和 63.6% 的胞外多糖产生。芽孢杆菌 HN-2 （HN-2E） 和杆菌肽正丁醇提取物对 ∆secG 的最低抑菌浓度 （MIC50） 值分别为 0.426 μg/mL（比 PXO99A 低 5.3%）和 10.905 μg/mL。值得注意的是，∆secG 对过氧化氢 （H2O2） 的敏感性增加，在 0.25 mm 时受到抑制，而 PXO99A 和 C：∆secG 为 0.3 mm。在 0.2 mM H2O2 存在下，∆secG 对 HN-2E 的敏感性增加了 31.22% （MIC50 = 0.159 μg/mL），而 PXO99A 和 C：∆secG 的 MIC50 值分别为 0.280 和 0.291 μg/mL。结论我们的研究结果表明，芽孢杆菌诱导的 H2O2 产生增强了米炎 X. oryzae pv 的敏感性。水稻到生物防治剂，为预防细菌性叶枯病提供有价值的见解。这些结果突出了 Sec 通路在米 X. oryzae pv 行为和抗性中的重要性。米佐，以及进一步研究植物病害的潜在领域。© 2024 化工学会.