Oxidative stress plays a crucial role in various pathological conditions. This study introduces an enhanced model using hydrogen peroxide (H2O2)-induced stress in Galleria mellonella larvae, offering a cost-effective and ethically sound alternative for oxidative stress research. The model bridges in vitro and in vivo studies to identify biomarkers like lipid peroxidation, protein carbonylation, hemocyte count, and antioxidant enzyme activities. Our results show that while G. mellonella larvae tolerated high doses of H2O2, increased susceptibility occurred with prolonged toxicosis and higher concentrations. Acute H2O2 exposure (5.0 M/1st day) led to elevated lipid and protein oxidation and decreased superoxide dismutase activity and hemocyte count, while catalase activity and total antioxidant capacity increased. Despite these defenses, the larvae's antioxidant capacity was insufficient under severe oxidative stress, reducing survival. This study highlights G. mellonella larvae as a promising model for examining reactive oxygen species (ROS)-induced oxidative stress.

中文翻译：

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利用 H2O2 诱导的 Galleria mellonella 幼虫的易感性：探索氧化应激和生物标志物的稳健模型


氧化应激在各种病理状况中起着至关重要的作用。本研究介绍了一种使用过氧化氢 （H2O2） 诱导的 Galleria mellonella 幼虫应激的增强模型，为氧化应激研究提供了一种经济高效且合乎道德的替代方案。该模型将体外和体内研究联系起来，以识别脂质过氧化、蛋白质羰基化、血细胞计数和抗氧化酶活性等生物标志物。我们的结果表明，虽然 G. mellonella 幼虫耐受高剂量的 H 2 O 2 ，但随着中毒时间的延长和浓度的增加，敏感性增加。急性 H 2 O 2 暴露 （5.0 M/第 1 天） 导致脂质和蛋白质氧化升高，超氧化物歧化酶活性和血细胞计数降低，而过氧化氢酶活性和总抗氧化能力增加。尽管有这些防御措施，但在严重的氧化应激下，幼虫的抗氧化能力不足，从而降低了存活率。本研究强调 G. mellonella 幼虫是检查活性氧 （ROS） 诱导的氧化应激的有前途的模型。