The complexity of regeneration extends beyond local wound responses, eliciting systemic processes across the entire organism. However, the functional relevance and coordination of distant molecular processes remain unclear. In the cnidarian Nematostella vectensis, we show that local regeneration triggers a systemic homeostatic response, leading to coordinated whole-body remodeling. Leveraging spatial transcriptomics, endogenous protein tagging, and live imaging, we comprehensively dissect this systemic response at the organismal scale. We identify proteolysis as a critical process driven by both local and systemic upregulation of metalloproteases. We show that metalloproteinase expression levels and activity scale with the extent of tissue loss. This proportional response drives long-range tissue and extracellular matrix movement. Our findings demonstrate the adaptive nature of the systematic response in regeneration, enabling the organism to maintain shape homeostasis while coping with a wide range of injuries.

中文翻译：

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海葵局部再生过程中全身组织重塑的系统协调


再生的复杂性超出了局部伤口反应，引发了整个生物体的系统过程。然而，远距离分子过程的功能相关性和协调性仍不清楚。在刺胞动物 Nematostella vectensis 中，我们表明局部再生会触发系统性稳态反应，导致协调的全身重塑。利用空间转录组学、内源性蛋白质标记和实时成像，我们在生物体尺度上全面剖析了这种系统反应。我们将蛋白水解确定为由金属蛋白酶的局部和全身上调驱动的关键过程。我们表明金属蛋白酶表达水平和活性随组织丢失的程度而变化。这种比例响应驱动组织和细胞外基质的长距离移动。我们的研究结果证明了再生中系统反应的适应性，使生物体能够在应对各种伤害的同时保持形状稳态。