The paper demonstrates an adaptation of a Prusa Mini+ 3D printer through the integration of 3D printed modules, creating a system capable of producing varied droplets from multiple Eppendorf tubes. Building upon our previous model, this system enhances calibration methodology enabling any fused deposition modeling (FDM) printer to produce mono-disperse droplets (coefficient of variance (CV%) <2% for train of 100 droplets) with 6900 assays per hour rate. The cost of the developed system is 85% lower than that of existing droplet generation solutions on the market, and 30% more economical than the previous iteration of the system. Additionally, the system's utility in quantification of agglutination assays is highlighted using image analysis, capable of distinguishing between agglutinated and non-agglutinated samples. By offering significant savings and ease of use, this system aims to lower the barriers to entry for microfluidic research, potentially broadening the scope of scientific exploration and application in this field.

中文翻译：

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自动动态入口微流体 （ADIM） 系统：经济高效的双轴纳升液滴按需生成平台及其在凝集分析中的应用。


该论文展示了 Prusa Mini+ 3D 打印机通过集成 3D 打印模块的改造，创建了一个能够从多个 Eppendorf 管中生产各种液滴的系统。该系统基于我们之前的模型，增强了校准方法，使任何熔融沉积建模 （FDM） 打印机都能以每小时 6900 次分析的速度产生单分散液滴（100 个液滴的系列方差系数 （CV%） <2%）。开发的系统成本比市场上现有的液滴发生解决方案低 85%，比之前的系统迭代经济 30%。此外，使用图像分析突出了该系统在凝集测定定量中的效用，能够区分凝集和非凝集样品。通过提供显着的成本节省和易用性，该系统旨在降低微流体研究的准入门槛，从而有可能拓宽该领域的科学探索和应用范围。