The intimate association between telomerase activity and cancer has driven the exploration of diverse methodologies for its precise detection. However, detecting telomerase activity at the single-cell level remains a significant challenge. Herein, we present a MOF-DNA barcode-amplified CRISPR-Cas12a strategy integrated with a single-cell microfluidic chip for ultrasensitive detection of telomerase activity. DNA-functionalized UiO-66 nanoparticles act as signal transducers, effectively converting telomerase activity into DNA activation strands, which subsequently trigger the trans-cleavage activity of CRISPR-Cas12a. This amplification-based assay could be integrated with a microfluidic chip to enable highly sensitive detection of telomerase activity at the single-cell level, offering promising advancements in early cancer diagnosis.

中文翻译：

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使用基于 CRISPR-Cas12a 的芯片在单细胞水平上检测端粒酶活性。


端粒酶活性与癌症之间的密切联系推动了对其精确检测的不同方法的探索。然而，在单细胞水平检测端粒酶活性仍然是一个重大挑战。在此，我们提出了一种 MOF-DNA 条形码扩增的 CRISPR-Cas12a 策略，该策略与单细胞微流控芯片集成，用于端粒酶活性的超灵敏检测。DNA 功能化的 UiO-66 纳米颗粒充当信号转导器，有效地将端粒酶活性转化为 DNA 激活链，随后触发 CRISPR-Cas12a 的反式切割活性。这种基于扩增的检测可以与微流控芯片集成，以实现在单细胞水平上高度灵敏地检测端粒酶活性，为早期癌症诊断提供有希望的进展。