BACKGROUNDHuanglongbing (HLB) is a systemic disease of citrus caused by the bacterial pathogen Candidatus Liberibacter asiaticus (CLas) that limits citrus production worldwide. CLas is an obligate bacterial pathogen that multiplies in citrus trees and in the insect vector, the Asian citrus psyllid (ACP), Diaphorina citri Kuwayama. There is no cure for HLB currently and broad‐spectrum antibiotics represent one possible therapeutic against disease symptoms. Single‐stranded nucleic acid analogs, 2′‐deoxy‐2’‐Fluoro‐β‐D‐arabinonucleic antisense oligonucleotides (FANA ASOs), can modulate gene expression by enzymatic degradation or steric blocking of an RNA target. FANA ASOs recognize and bind to specific RNA forms, including mRNA, miRNA and long noncoding RNA, through complementary base‐pairing.RESULTSInjection of oxytetracycline (OTC) into mature citrus trees with HLB ameliorated symptoms of disease, increased fruit yield and quality of juice as compared with that produced by noninjected controls. Injection of trees with FANA ASOs reduced CLas abundance but did not improve fruit yield and quality above control levels at the injection dosage tested. Reduced pathogen relative abundance following OTC injection was coincident with lower CLas acquisition and inoculation by laboratory deployed and wild‐type D. citri collected from the field, respectively. However, injections of FANA ASOs did not consistently reduce CLas transmission as compared with noninjected controls.CONCLUSIONSTrunk injection of OTC may be useful in management of HLB by reducing CLas infection in trees and disrupting transmission. However, more research is needed to verify the potential usefulness of FANA ASOs. Optimizing FANA ASO dosage in trees and exploring the potential of multiplex FANA ASOs that simultaneously target multiple mRNAs could possibly enhance their efficacy against CLas. © 2024 Society of Chemical Industry.

中文翻译：

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树注射土霉素和靶向柑橘中 Candidatus Liberibacter asiaticus 的反义寡核苷酸的评价


背景搜龙病 （HLB） 是一种由细菌病原体亚洲假丝酵母菌 （CLas） 引起的柑橘系统性疾病，限制了全球柑橘的生产。CLas 是一种专性细菌病原体，在柑橘树和昆虫媒介亚洲柑橘木虱 （ACP） Diaphorina citri Kuwayama 中繁殖。目前尚无治愈 HLB 的方法，广谱抗生素是针对疾病症状的一种可能治疗方法。单链核酸类似物，2'-脱氧-2'-氟-β-D-阿拉伯核酸反义寡核苷酸 （FANA ASO），可以通过酶降解或空间阻断 RNA 靶标来调节基因表达。FANA ASO 通过互补碱基配对识别并结合特定 RNA 形式，包括 mRNA、miRNA 和长链非编码 RNA。结果与未注射对照相比，将土霉素 （OTC） 注射到具有 HLB 的成熟柑橘树中可改善疾病症状，提高果实产量和果汁质量。在测试的注射剂量下，用 FANA ASO 注射树木降低了 CLas 的丰度，但并没有提高果实产量和质量，高于对照水平。OTC 注射后病原体相对丰度降低与实验室部署和从田间收集的野生型 D. citri 的较低 CLas 获取和接种相吻合。然而，与未注射的对照相比，注射 FANA ASO 并未始终减少 CLas 传播。结论 OTC 注射可能有助于减少树木 CLas 感染和阻断传播，从而有助于管理 HLB。然而，需要更多的研究来验证 FANA ASO 的潜在有用性。 优化树木中的 FANA ASO 剂量并探索同时靶向多个 mRNA 的多重 FANA ASO 的潜力可能会增强它们对 CLas 的疗效。© 2024 化工学会.