Plants demonstrate a high degree of developmental plasticity, capable of regenerating entire individuals from detached somatic tissues—a regenerative phenomenon rarely observed in metazoa. Consequently, elucidating the lineage relationship between somatic founder cells and descendant cells in regenerated plant organs has long been a pursuit. In this study, we developed and optimized both DNA barcode- and multi-fluorescence-based cell-lineage tracing toolsets, employing an inducible method to mark individual cells in Arabidopsis donor somatic tissues at the onset of regeneration. Utilizing these complementary methods, we scrutinized cell identities at the single-cell level and presented compelling evidence that all cells in the regenerated Arabidopsis plants, irrespective of their organ types, originated from a single progenitor cell in the donor somatic tissue. Our discovery suggests a single-cell passage directing the transition from multicellular donor tissue to regenerated plants, thereby creating opportunities for cell-cell competition during plant regeneration—a strategy for maximizing survival.

中文翻译：

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开发诱导型 DNA 条形码系统以了解拟南芥再生中的谱系变化


植物表现出高度的发育可塑性，能够从分离的体细胞组织中再生整个个体——这种再生现象在后生动物中很少见。因此，阐明再生植物器官中体细胞创始细胞和后代细胞之间的谱系关系长期以来一直是一项追求。在这项研究中，我们开发并优化了基于 DNA 条形码和多荧光的细胞谱系追踪工具集，采用诱导方法在再生开始时标记拟南芥供体组织中的单个细胞。利用这些互补方法，我们在单细胞水平上仔细检查了细胞身份，并提供了令人信服的证据，证明再生拟南芥植物中的所有细胞，无论其器官类型如何，都起源于供体体组织中的单个祖细胞。我们的发现表明，单细胞传代引导从多细胞供体组织过渡到再生植物，从而在植物再生过程中为细胞间竞争创造机会——这是一种最大限度地提高存活率的策略。