Aminoluciferins Extend Firefly Luciferase Bioluminescence into the Near-Infrared and Can Be Preferred Substrates overd-Luciferin,Journal of the American Chemical Society

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Aminoluciferins Extend Firefly Luciferase Bioluminescence into the Near-Infrared and Can Be Preferred Substrates overd-Luciferin
Journal of the American Chemical Society ( IF 14.4 ) Pub Date : 2014-09-11 , DOI: 10.1021/ja505795s
David M Mofford ₁ , Gadarla Randheer Reddy , Stephen C Miller

Affiliation

Firefly luciferase adenylates and oxidizes d-luciferin to chemically generate visible light and is widely used for biological assays and imaging. Here we show that both luciferase and luciferin can be reengineered to extend the scope of this light-emitting reaction. d-Luciferin can be replaced by synthetic luciferin analogues that increase near-infrared photon flux >10-fold over that of d-luciferin in live luciferase-expressing cells. Firefly luciferase can be mutated to accept and utilize rigid aminoluciferins with high activity in both live and lysed cells yet exhibit 10 000-fold selectivity over the natural luciferase substrate. These new luciferin analogues thus pave the way to an extended family of bioluminescent reporters.

中文翻译：

氨基荧光素将萤火虫荧光素酶的生物发光扩展到近红外区域，可以作为首选底物过荧光素

萤火虫荧光素酶腺苷酸化和氧化 d-荧光素以化学方式产生可见光，广泛用于生物测定和成像。在这里，我们表明荧光素酶和荧光素都可以重新设计以扩展这种发光反应的范围。d-荧光素可以被合成荧光素类似物替代，在表达荧光素酶的活细胞中，近红外光子通量是 d-荧光素通量的 10 倍以上。萤火虫荧光素酶可以突变以接受和利用在活细胞和裂解细胞中都具有高活性的刚性氨基荧光素，但其选择性比天然荧光素酶底物高 10 000 倍。因此，这些新的荧光素类似物为生物发光报告基因的扩展家族铺平了道路。

更新日期：2014-09-11

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