In grape breeding programs, the extensive planting of seedlings is a crucial aspect. However, grape seeds display distinct dormancy traits, necessitating a prolonged cold stratification process for dormancy release. In order to enhance the efficiency of breeding programs, this study presents an innovative in vitro embryo germination technique that eliminates the requirement for cold stratification of seeds. The method involves the disruption of peripheral tissue in grape seed embryos using a straightforward mechanical technique, resulting in the efficient production of a substantial quantity of seed embryos，with a germination rate of up to 88 % for these isolated embryos. These embryos are subsequently cultured in vitro to facilitate germination into seedlings, thereby eliminating the need for cold stratification. Consequently, grape seedlings can be obtained within a significantly reduced timeframe of 30–38 d, expediting the overall grape breeding process. This novel approach not only accelerates grape hybridization but also streamlines the selection of new grape varieties, contributing to an efficient and time-sensitive breeding methodology.

中文翻译：

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通过体外胚胎萌发技术推进葡萄育种，无需冷分层


在葡萄育种计划中，幼苗的广泛种植是一个至关重要的方面。然而，葡萄种子表现出明显的休眠特性，需要长时间的冷分层过程才能释放休眠。为了提高育种计划的效率，本研究提出了一种创新的 体外胚胎发芽技术，该技术消除了种子冷分层的要求。该方法涉及使用简单的机械技术破坏葡萄种子胚胎中的外周组织，从而有效地生产大量种子胚胎，这些分离胚胎的发芽率高达 88%。这些胚胎随后在 体外培养，以促进发芽成幼苗，从而消除冷分层的需要。因此，可以在 30-38 天的显著缩短时间内获得葡萄幼苗，从而加快了整个葡萄育种过程。这种新方法不仅加速了葡萄杂交，还简化了新葡萄品种的选择，有助于实现高效且对时间敏感的育种方法。