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Comparative analysis of fecal microbiota between diarrhea and non-diarrhea piglets reveals biomarkers of gut microbiota associated with diarrhea
Animal Nutrition ( IF 6.1 ) Pub Date : 2024-09-28 , DOI: 10.1016/j.aninu.2024.05.013
Jiang Zhu, Yue Sun, Lingyan Ma, Qu Chen, Caihong Hu, Hua Yang, Qihua Hong, Yingping Xiao

Diarrhea poses a significant threat to the health and well-being of weaned piglets, leading to substantial morbidity and mortality and economic loss in the pig industry. However, the structural characteristics of the gut microbiota and the key genera associated with early diarrhea in piglets within large-scale production systems are poorly understood. This study aimed to investigate the differences in the microbial community structure and the specific genera alteration between the healthy piglets and diarrhea piglets, and to identify the biomarkers of gut microbiota associated with diarrhea in piglets. A total of 250 fecal samples, including 130 healthy piglets (Duroc × Landrace × Large Yorkshire) in the Control group and 120 from diarrhea piglets in Diarrhea group, were collected from three large-scale farms as discovery cohorts and were used for 16S rRNA gene sequencing. Additionally, 150 fecal samples from another large-scale pig farm were collected for the validation trail. The Chao1 and ACE indices were obviously lower (P < 0.01) in the diarrhea piglets compared to the healthy ones. Principal coordinate analysis showed significant differences in the distance matrix of gut microbiota between the healthy and diarrhea piglets (Bray-Curtis: P = 0.001, Jaccard: P = 0.001). Eighty-five genera were differentially enriched (P < 0.001) between healthy and diarrhea piglets. Notably, Treponema, Sphaerochaeta, Escherichia-Shigella, Slackia, and Staphylococcus were identified as potential biomarkers of diarrhea susceptibility; Clostridium sensu stricto 1, Prevotella_9, Olsenella, Dorea, and Lachnospiraceae NK4A136 group were found to be beneficial for maintaining intestinal homeostasis. These differentially enriched genera of healthy and diarrhea piglets were further confirmed in the validation cohort. In conclusion, this study identified the diarrhea-associated and beneficial genera in the faces of piglet, providing a theoretical basis for the diagnosis and intervention of diarrhea in weaned piglets.

中文翻译:


腹泻仔猪和非腹泻仔猪粪便微生物群的比较分析揭示了与腹泻相关的肠道微生物群的生物标志物



腹泻对断奶仔猪的健康和福祉构成重大威胁,导致养猪业的发病率和死亡率以及经济损失。然而,在大规模生产系统中,肠道微生物群的结构特征和与仔猪早期腹泻相关的关键属知之甚少。本研究旨在探讨健康仔猪和腹泻仔猪之间微生物群落结构和特异性属改变的差异,并确定与仔猪腹泻相关的肠道菌群生物标志物。从三个大型猪场收集 250 份粪便样本,包括对照组 130 头健康仔猪 (杜洛克×长白 × 大约克郡) 和腹泻组 120 头腹泻仔猪 作为发现队列,用于 16S rRNA 基因测序。此外,还从另一个大型养猪场收集了 150 个粪便样本用于验证跟踪。与健康仔猪相比,腹泻仔猪的 Chao1 和 ACE 指数明显降低 (P < 0.01)。主坐标分析显示健康仔猪和腹泻仔猪之间肠道菌群的距离矩阵存在显著差异(Bray-Curtis:P = 0.001,Jaccard:P = 0.001)。85 个属在健康仔猪和腹泻仔猪之间差异富集 (P < 0.001)。值得注意的是,密螺旋体、球形毛壳体、埃希氏菌志贺氏菌、松弛菌和葡萄球菌被确定为腹泻易感性的潜在生物标志物;发现严格意义梭菌 1、Prevotella_9、Olsenella、Dorea 和 Lachnospiraceae NK4A136组有益于维持肠道稳态。 这些差异富集的健康仔猪和腹泻仔猪的属在验证队列中得到进一步证实。综上所述,本研究确定了仔猪面部腹泻相关和有益菌属,为断奶仔猪腹泻的诊断和干预提供了理论依据。
更新日期:2024-09-28
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