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Compound Bacillus improves eggshell quality and egg metabolites of hens by promoting the metabolism balance of calcium and phosphorus and uterine cell proliferation
Animal Nutrition ( IF 6.1 ) Pub Date : 2024-08-18 , DOI: 10.1016/j.aninu.2024.04.023
Qian Jin, Fei Wang, Weisheng Ye, Qi Wang, Shujie Xu, Shaoxiong Jiang, Xiang Li, Min Yue, Dongyou Yu, Mingliang Jin, Aikun Fu, Weifen Li
Animal Nutrition ( IF 6.1 ) Pub Date : 2024-08-18 , DOI: 10.1016/j.aninu.2024.04.023
Qian Jin, Fei Wang, Weisheng Ye, Qi Wang, Shujie Xu, Shaoxiong Jiang, Xiang Li, Min Yue, Dongyou Yu, Mingliang Jin, Aikun Fu, Weifen Li
Probiotics have beneficial effects on improving egg quality, but there is little research about the effect of probiotics on metabolite composition, and the mechanisms are not yet fully understood. The aim of this study was to investigate the potential mechanisms by which compound Bacillus improves egg quality and metabolite composition. A total of 20,000 Jingfen No. 6 laying hens at 381 d old were randomly divided into two treatments: control group with a basal diet, and the basal diet with 5 × 108 CFU/kg compound Bacillus supplementation (Ba) group. The trial lasted eight weeks. The results showed that compound Bacillus improved the gloss and strength of eggshells and reduced the ratio of sand-shell eggs by 23.8%. Specifically, the effective layer of eggshell was thicker and its calcite column was closely connected. Compound Bacillus increased the contents of beneficial fatty acids in the egg yolk, and lipids and lipid-like molecules in the albumen (P < 0.01), while decreased the contents of total cholesterol, triglycerides, and benzene ring compounds in the egg yolk and organic oxygen compounds in the albumen (P < 0.01). In addition, the compound Bacillus increased the calcium absorption in the duodenum by up-regulating the expression of transporters and serum hormone synergism (P < 0.05), and promoted metabolic balance of calcium and phosphorus. Simultaneously, uterine transcriptome showed that the expression of ChaC glutathione specific gamma-glutamylcyclotransferase 1 (CHAC1 ), glycoprotein-N-acetylgalactosamine 3-beta-galactosyltransferase 1 (C1GALT1 ), phosphatidylinositol-4-phosphate 5-kinase type 1 beta (PIP5K1B ), methylenetetrahydrofolate dehydrogenase 2 (MTHFD2 ), brain enriched myelin associated protein 1 (BCAS1 ), and squalene epoxidase (SQLE) genes were increased (P < 0.01), indicating that nutrient metabolism activity was enhanced. The expression of the BCAS1, C1GALT1, KLF transcription factor 13 (KLF13 ), and leucine rich repeat neuronal 1 (LRRN1 ) was increased (P < 0.01), indicating that the cell proliferation was enhanced, which slowed uterus aging. In conclusion, compound Bacillus improved the eggshell strength and metabolite composition in the egg by promoting metabolic balance of calcium and phosphorus, cell proliferation, and nutrient metabolism in the uterus.
中文翻译:
复合芽孢杆菌通过促进钙磷代谢平衡和子宫细胞增殖来改善蛋壳品质和鸡蛋代谢产物
益生菌对提高鸡蛋质量有有益的作用,但关于益生菌对代谢物组成的影响的研究很少,其机制尚不完全清楚。本研究的目的是研究化合物芽孢杆菌改善鸡蛋质量和代谢物组成的潜在机制。将 20 000 只 381 d 龄景分 6 号蛋鸡随机分为 2 个处理:对照组(基础日粮组)和 5 × 108 CFU/kg 复合芽孢杆菌补充 (Ba) 的基础日粮组。审判持续了八周。结果表明,复合芽孢杆菌提高了蛋壳的光泽和强度,使沙壳蛋的比例降低了 23.8%。具体而言,蛋壳有效层较厚,其方解石柱连接紧密。复合芽孢杆菌增加了蛋黄中有益脂肪酸的含量,蛋白中脂质和脂质样分子的含量(P < 0.01),同时降低了蛋黄中总胆固醇、甘油三酯和苯环化合物的含量,以及蛋白中有机氧化合物的含量(P < 0.01)。此外,复方芽孢杆菌通过上调转运蛋白的表达和血清激素协同作用增加十二指肠对钙的吸收 (P < 0.05),促进钙磷的代谢平衡。 同时,子宫转录组显示 ChaC 谷胱甘肽特异性 γ-谷氨酰环转移酶 1 (CHAC1)、糖蛋白-N-乙酰半乳糖胺 3-β-半乳糖基转移酶 1 (C1GALT1)、磷脂酰肌醇-4-磷酸 5-激酶 1 型 β (PIP5K1B)、亚甲基四氢叶酸脱氢酶 2 (MTHFD2) 、脑富集髓鞘相关蛋白 1 (BCAS1) 和角鲨烯环氧化物酶 (SQLE) 基因的表达升高 (P < 0.01),表明营养代谢活性增强。BCAS1、C1GALT1、KLF 转录因子 13 (KLF13) 和亮氨酸富集重复神经元 1 (LRRN1) 的表达增加 (P < 0.01),表明细胞增殖增强,从而减缓子宫衰老。综上所述,复合芽孢杆菌通过促进子宫内钙磷代谢平衡、细胞增殖和营养代谢,改善卵子中的蛋壳强度和代谢物组成。
更新日期:2024-08-18
中文翻译:
复合芽孢杆菌通过促进钙磷代谢平衡和子宫细胞增殖来改善蛋壳品质和鸡蛋代谢产物
益生菌对提高鸡蛋质量有有益的作用,但关于益生菌对代谢物组成的影响的研究很少,其机制尚不完全清楚。本研究的目的是研究化合物芽孢杆菌改善鸡蛋质量和代谢物组成的潜在机制。将 20 000 只 381 d 龄景分 6 号蛋鸡随机分为 2 个处理:对照组(基础日粮组)和 5 × 108 CFU/kg 复合芽孢杆菌补充 (Ba) 的基础日粮组。审判持续了八周。结果表明,复合芽孢杆菌提高了蛋壳的光泽和强度,使沙壳蛋的比例降低了 23.8%。具体而言,蛋壳有效层较厚,其方解石柱连接紧密。复合芽孢杆菌增加了蛋黄中有益脂肪酸的含量,蛋白中脂质和脂质样分子的含量(P < 0.01),同时降低了蛋黄中总胆固醇、甘油三酯和苯环化合物的含量,以及蛋白中有机氧化合物的含量(P < 0.01)。此外,复方芽孢杆菌通过上调转运蛋白的表达和血清激素协同作用增加十二指肠对钙的吸收 (P < 0.05),促进钙磷的代谢平衡。 同时,子宫转录组显示 ChaC 谷胱甘肽特异性 γ-谷氨酰环转移酶 1 (CHAC1)、糖蛋白-N-乙酰半乳糖胺 3-β-半乳糖基转移酶 1 (C1GALT1)、磷脂酰肌醇-4-磷酸 5-激酶 1 型 β (PIP5K1B)、亚甲基四氢叶酸脱氢酶 2 (MTHFD2) 、脑富集髓鞘相关蛋白 1 (BCAS1) 和角鲨烯环氧化物酶 (SQLE) 基因的表达升高 (P < 0.01),表明营养代谢活性增强。BCAS1、C1GALT1、KLF 转录因子 13 (KLF13) 和亮氨酸富集重复神经元 1 (LRRN1) 的表达增加 (P < 0.01),表明细胞增殖增强,从而减缓子宫衰老。综上所述,复合芽孢杆菌通过促进子宫内钙磷代谢平衡、细胞增殖和营养代谢,改善卵子中的蛋壳强度和代谢物组成。