The stable lignocellulose structure in the straw is the main obstacle for methane production during its anaerobic digestion, and the residual chlorophenols in the straw further increase the difficulty. In this study, the anaerobic digestion of corn straw containing 4-chlorophenol was enhanced by the addition of Phanerochaete chrysosporium and biochar. The results revealed that P. chrysosporium significantly increased the soluble COD concentration and total COD removal efficiency in the anaerobic digestion of corn straw, which initially contained a small amount of residual oxygen (4.1–4.5 mg/L). The accumulative methane production of the P. chrysosporium-coupled biochar (PC-BC) group and the PC group with P. chrysosporium alone were 232.9 ± 3.0 mL and 201.7 ± 5.1 mL, respectively, which were significantly higher than the control group (19.4 ± 1.0 mL) with the sterilized P. chrysosporium. The presence of biochar increased 4-CP removal rate to 93.3 %, which was 15.2 % higher than the control. Additionally, FTIR analysis indicated that the addition of P. chrysosporium and biochar enhanced the decomposition of lignocellulose structure. Moreover, the sludge capacitance and electron transfer capacity were highest in the PC-BC group. Also, microbial community analysis showed that biochar could enrich dechlorinating bacteria (e.g., Sedimentibacter) and electroactive microorganisms, which further enhanced dechlorination and methanogensis.

中文翻译：

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通过在微需氧条件下添加 Phanerochaete chrysosporium 和生物炭来提高玉米秸秆厌氧消化中的甲烷产生和 4-氯苯酚去除


秸秆中稳定的木质纤维素结构是其厌氧消化过程中产生甲烷的主要障碍，秸秆中残留的氯酚进一步增加了难度。在本研究中，添加 Phanerochaete chrysosporium 和生物炭增强了含 4-氯苯酚的玉米秸秆的厌氧消化。结果表明，黄孢假单胞菌显著提高了玉米秸秆厌氧消化过程中可溶性 COD 浓度和总 COD 去除效率，玉米秸秆最初含有少量残氧 （4.1–4.5 mg/L）。黄孢松偶联生物炭 （PC-BC） 组和单独使用金孢松的 PC 组的累积甲烷产量分别为 232.9 ± 3.0 mL 和 201.7 ± 5.1 mL，显著高于使用绝育黄孢松的对照组 （19.4 ± 1.0 mL）。生物炭的存在将 4-CP 去除率提高到 93.3 %，比对照高 15.2 %。此外，FTIR 分析表明，P. chrysosporium 和生物炭的添加增强了木质纤维素结构的分解。此外，PC-BC 组的污泥电容和电子转移容量最高。此外，微生物群落分析表明，生物炭可以富集脱氯细菌（例如 Sedimentibacter）和电活性微生物，从而进一步增强脱氯和产甲烷菌。