ObjectiveThe objective of this study is to compare the effect of the location of recombinant human bone morphogenetic protein 2 (rhBMP‐2) from the native bone and the periosteum for vertical alveolar bone augmentation.Materials and MethodsMandibular, chronic, standardized, bilateral, and vertical defects in 12 beagle dogs were evaluated using four modalities: a xenograft alone (XENO; n = 6); rhBMP‐2 alone (BMP; n = 6); a technique with rhBMP‐2 close to the host bone covered by xenograft (SAN; n = 6); and a technique with rhBMP‐2 close to the flap on top of the xenograft (LAS; n = 6). After 8 weeks, a series of in vivo inspections, fluorescence microscopy, histologic and histomorphometric evaluations, and micro‐CT analyses.ResultsAfter 8 weeks of healing, new bone formation correlated with proximity of rhBMP to the perforated membrane with BMP and LAS (p = 0.024). The highest total bone volume was found in the LAS group (45.1% ± 13.3%), followed by the SAN group (35.2% ± 6.7%), BMP group (33.1% ± 11.8%), followed by the XENO group (23.1% ± 6.5%). The SAN group demonstrated frequent seroma formation. Blood vessel formation was more pronounced in the LAS + rhBMP group, with a significant increase of 27.1% compared to the XENO group (p = 0.02). Micro‐CT revealed a strong trend for higher bone volume in the BMP group (34.7%) compared to the XENO group (13.6%) (p = 0.06). Only rhBMP‐2 groups demonstrated bone formation above the perforated membrane.ConclusionThe location of rhBMP‐2 in relation to the biomaterial and periosteum influenced the effectiveness of vertical bone regeneration.

中文翻译：

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使用微量 rhBMP-2 再生慢性牙槽垂直缺损。一项实验性体内研究


目的比较天然骨和骨膜重组人骨形态发生蛋白 2 （rhBMP-2） 定位对垂直牙槽骨增强的影响。材料和方法使用四种方式评估 12 只比格犬的下颌、慢性、标准化、双侧和垂直缺损：单独异种移植 （XENO;n = 6）;单独使用 rhBMP-2 （BMP;n = 6）;一种 rhBMP-2 靠近异种移植物覆盖的宿主骨的技术 （SAN;n = 6）;以及一种 rhBMP-2 靠近异种移植物顶部皮瓣的技术 （LAS;n = 6）。8 周后，进行一系列体内检查、荧光显微镜检查、组织学和组织形态学评估以及显微 CT 分析。结果愈合 8 周后，新骨形成与 rhBMP 与 BMP 和 LAS 穿孔膜的接近程度相关 （p = 0.024）。LAS 组 （45.1% ± 13.3%） 的总骨体积最高，其次是 SAN 组 （35.2% ± 6.7%）、BMP 组 （33.1% ± 11.8%），其次是 XENO 组 （23.1% ± 6.5%）。SAN 组表现出频繁的血清肿形成。LAS + rhBMP 组血管形成更为明显，与 XENO 组相比显着增加 27.1% （p = 0.02）。显微 CT 显示，与 XENO 组 （13.6%） 相比，BMP 组 （34.7%） 的骨量呈较高趋势 （p = 0.06）。只有 rhBMP-2 组在穿孔膜上方表现出骨形成。结论rhBMP-2 相对于生物材料和骨膜的位置影响垂直骨再生的有效性。