Aims

Microbial inoculants play important roles in the remediation of abandoned mine sites by stimulating plant growth. However, further investigations are required to elucidate their impacts on soil microbial communities and metabolites in this process.

Methods

For this study, we employed amplicon sequencing and Liquid Chromatography-Tandem Mass Spectrometry (LC–MS/MS) techniques to examine the responses of soil microbial communities and metabolic functions to microbial inoculants using a pot experiment.

Results

Our findings indicated that microbial inoculants significantly influenced the structures of both bacterial and fungal communities. Network analyses revealed that microbial inoculants enhanced the stability and complexity of bacterial co-occurrence networks while reducing those of their fungal counterparts. Moreover, microbial inoculants increased the importance of stochastic processes in community assembly, particularly within fungal communities. Metabolomic analyses showed that microbial inoculants significantly modified the metabolic profiles of rhizospheric soil and upregulated metabolic pathways of phenylpropanoids and isoflavonoids. Correlation analyses and structural equation modeling (SEM) analysis further showed that microbial inoculants promote plant growth by influencing keystone microbial genera and the metabolism of phenylpropanoids and isoflavonoids.

Conclusion

Overall, this study demonstrated that microbial inoculants promote plant growth through the modification of rhizospheric keystone microbial taxa and the metabolism of phenylpropanoids and isoflavonoids. These results offer valuable insights into the complex biological processes influenced by microbial inoculants in the rhizosphere.

中文翻译：

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微生物接种剂诱导的根际代谢物和微生物群落的修饰促进了植物生长

 目标

微生物接种剂通过刺激植物生长，在废弃矿区的修复中发挥着重要作用。然而，需要进一步研究以阐明它们在此过程中对土壤微生物群落和代谢物的影响。

 方法

在这项研究中，我们采用扩增子测序和液相色谱-串联质谱 （LC-MS/MS） 技术，通过罐栽实验检查土壤微生物群落和代谢功能对微生物接种剂的响应。

 结果

我们的研究结果表明，微生物接种剂显着影响细菌和真菌群落的结构。网络分析表明，微生物接种剂增强了细菌共生网络的稳定性和复杂性，同时减少了真菌共生网络的稳定性和复杂性。此外，微生物接种剂增加了随机过程在群落组装中的重要性，尤其是在真菌群落中。代谢组学分析表明，微生物接种剂显着改变了根际土壤的代谢谱，并上调了苯丙烷类和异黄酮类化合物的代谢途径。相关性分析和结构方程模型 （SEM） 分析进一步表明，微生物接种剂通过影响关键微生物属以及苯丙烷类和异黄酮类化合物的代谢来促进植物生长。

 结论

总体而言，这项研究表明，微生物接种剂通过修饰根际基石微生物类群以及苯丙烷类化合物和异黄酮类化合物的代谢来促进植物生长。这些结果为了解根际微生物接种剂影响的复杂生物过程提供了有价值的见解。