We identified a novel mastadenovirus, herein referred to as colobus adenovirus (CoAdV), as the likely cause of fatal respiratory and enteric diseases in multiple black-and-white colobuses (Colobus guereza) imported into Thailand in 2022. Among 9 affected colobuses, 4 died. Viral antigen was abundant in respiratory and enteric tissues, where prominent lesions and clinical signs were observed. We successfully cultivated CoAdV in Vero cells and characterized the complete viral genome, which indicated the virus is genetically distinct from other simian adenoviruses. We also conducted a retrospective study of archival samples from 7 other unrelated colobuses that had respiratory distress or diarrhea and found similar viral strains in 4 of those colobuses. Although we could not determine the potential harm to humans or other nonhuman primates from current information, the zoonotic and spillover potential of this virus to other related hosts should not be neglected. Veterinarians should consider CoAdV when pneumonia is diagnosed in colobuses.

我们确定了一种新型乳腺病毒，本文称为大肺疣腺病毒 （CoAdV），是 2022 年进口到泰国的多种黑白大叶 （Colobus guereza） 中致命呼吸道和肠道疾病的可能原因。在 9 例受影响的 colob 中，4 例死亡。病毒抗原在呼吸道和肠道组织中丰富，观察到明显的病变和临床体征。我们成功地在 Vero 细胞中培养了 CoAdV 并表征了完整的病毒基因组，这表明该病毒在遗传上与其他猿猴腺病毒不同。我们还对来自 7 个其他无关的肺叶的档案样本进行了一项回顾性研究，这些肺叶有呼吸窘迫或腹泻，并在其中 4 个肺叶中发现了类似的病毒株。尽管我们无法从当前信息中确定对人类或其他非人类灵长类动物的潜在危害，但不应忽视这种病毒的人畜共患和对其他相关宿主的溢出潜力。当诊断出结肠叶肺炎时，兽医应考虑 CoAdV。