Lycopi herba, a traditional Chinese medicinal plant, has long been valued for its aerial parts. however, its roots, which are often discarded as non-medicinal waste, actually contain flavonoid compounds that possess potential medicinal values such as anti-inflammatory, antioxidant, and anti-tumor activities. Despite this, studies on the extraction, purification, and biological activity assessment of total flavonoids from L. herba roots (TFLHR) remain inadequate. Our study aimed to optimize the extraction and purification processes for TFLHR and evaluate their anti-proliferative effects on human fibroblast-like synoviocytes (HFLS-RA), which are key pathological cells in rheumatoid arthritis. By utilizing ultrasound-assisted extraction combined with response surface methodology (RSM), we optimized the extraction conditions, achieving a total flavonoid content of 90.484 ± 0.974 mg/g under the optimal settings: a liquid–solid ratio of 48:1 mL/g, 13 min of ultrasound treatment, 70 % ethanol, and an extraction temperature of 43°C. Subsequently, macroporous resin chromatography was employed for flavonoid purification, with AB-8 resin exhibiting the highest performance, achieving adsorption and desorption rates of 79.64 ± 1.51 % and 88.61 ± 1.02 %, respectively. By further refining the purification conditions through RSM, the purity of flavonoids was increased to 63.9 ± 1.86 %. Through ultra performance liquid chromatography tandem-quadrupole time-of-flight tandem mass spectrometry (UPLC-Q-TOF-MS/MS) analysis, 74 flavonoid compounds across 15 categories were identified. Further activity studies demonstrated that purified TFLHR exhibited significant concentration-dependent anti-proliferative effects on HFLS-RA cells. This study not only provides a scientific basis for the comprehensive utilization of L. herba root resources but also highlights the potential medicinal value of TFLHR in the treatment of rheumatoid arthritis, laying a foundation for future research into its specific mechanisms and clinical applications.

中文翻译：

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石松根总黄酮提取纯化工艺优化及其对人类风湿性关节炎纤维滑膜细胞的抗增殖作用的研究


石蒜草是一种传统的中药植物，长期以来一直因其地上部分而受到重视。然而，它的根经常作为非药用废物丢弃，实际上含有具有潜在药用价值的类黄酮化合物，例如抗炎、抗氧化和抗肿瘤活性。尽管如此，关于从乳杆菌根中提取、纯化和生物活性评估总黄酮 （TFLHR） 的研究仍然不充分。我们的研究旨在优化 TFLHR 的提取和纯化过程，并评估它们对人成纤维细胞样滑膜细胞 （HFLS-RA） 的抗增殖作用，HFLS-RA 是类风湿性关节炎的关键病理细胞。通过超声辅助提取结合响应面法 （RSM），我们优化了提取条件，在液固比为 48：1 mL/g、超声处理 13 min、乙醇 70 % 、提取温度为 43°C 的最佳设置下，总黄酮含量为 90.484 ± 0.974 mg/g。 随后，采用大孔树脂色谱法进行类黄酮纯化，其中 AB-8 树脂表现出最高性能，吸附率和解吸率分别为 79.64 ± 1.51 % 和 88.61 ± 1.02 %。通过RSM进一步精制纯化条件，黄酮类化合物的纯度提高到63.9 ± 1.86 %。通过超高效液相色谱串联-四极杆飞行时间串联质谱 （UPLC-Q-TOF-MS/MS） 分析，鉴定出 15 类 74 种类黄酮化合物。进一步的活性研究表明，纯化的 TFLHR 对 HFLS-RA 细胞表现出显着的浓度依赖性抗增殖作用。 本研究不仅为乳杆菌根资源的综合利用提供了科学依据，而且突出了 TFLHR 治疗类风湿关节炎的潜在药用价值，为未来研究其具体机制和临床应用奠定了基础。