The advantages of simple structure and easy portability inherent to self-powered biosensors have broad application prospects in clinical diagnosis and implantable medical devices. In light of the limitations of the clinical detection sensitivity, this study devised and fabricated a three-dimensional (3D) cubic structure of MoS₂ that was self-assembled by 2D nanosheets. The multi-dimensional structure design provides a broad binding site for the incubation of biological probes and the loading of biological enzymes, which can accelerate the electron transfer rate and effectively improve the detection sensitivity. On this basis, a self-powered biosensor platform was constructed for the detection of miRNA-499, employing the catalytic hairpin self-assembly (CHA) signal amplification strategy. This strategy has the potential to enhance the accuracy of detection and circumvent the occurrence of false positive results. The experimental results demonstrate that the self-powered biosensor exhibits a detection limit (LOD) of 0.12 fmol/L (S/N = 3) within a wide linear range of 1 fmol/L to 100 pmol/L. Furthermore, the sensor platform exhibits excellent specificity, stability, and reproducibility. Moreover, the test signal is transmitted via Bluetooth to the smartphone interface, thus meeting the requirements for portable, real-time detection.

中文翻译：

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基于立方纳米结构和级联扩增策略的集成自供电传感器


自供电生物传感器固有的结构简单、易于携带等优点在临床诊断和植入式医疗器械中具有广阔的应用前景。鉴于临床检测灵敏度的局限性，本研究设计并制造了一种由 2D 纳米片自组装的 MoS₂ 三维 （3D） 立方结构。多维结构设计为生物探针的孵育和生物酶的加载提供了广阔的结合位点，可以加快电子转移速率，有效提高检测灵敏度。在此基础上，构建了用于检测 miRNA-499 的自供电生物传感器平台，采用催化发夹自组装 （CHA） 信号放大策略。此策略有可能提高检测的准确性并避免假阳性结果的发生。实验结果表明，自供电生物传感器在 1 fmol/L 至 100 pmol/L 的宽线性范围内表现出 0.12 fmol/L （S/N = 3） 的检测限 （LOD）。此外，该传感器平台表现出优异的特异性、稳定性和可重复性。此外，测试信号通过蓝牙传输到智能手机界面，从而满足便携式实时检测的要求。