Sequencing the monoclonal antibody variable regions using multiple charge integration middle-down strategy and ultraviolet photodissociation,Analytica Chimica Acta

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Sequencing the monoclonal antibody variable regions using multiple charge integration middle-down strategy and ultraviolet photodissociation
Analytica Chimica Acta ( IF 5.7 ) Pub Date : 2024-11-19 , DOI: 10.1016/j.aca.2024.343450
Jialiang Liu ₁ , Zheyi Liu ₂ , Heng Zhao ₃ , Chunlei Xiao ₄ , Xueming Yang ₅ , Fangjun Wang ₂

Affiliation

School of Pharmacy, China Medical University, Shenyang, 110122, China; CAS Key Laboratory of Separation Sciences for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, China; State Key Laboratory of Molecular Reaction Dynamics, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, China.
CAS Key Laboratory of Separation Sciences for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, China; State Key Laboratory of Molecular Reaction Dynamics, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, China; University of Chinese Academy of Sciences, Beijing, 100049, China.
CAS Key Laboratory of Separation Sciences for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, China; State Key Laboratory of Molecular Reaction Dynamics, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, China.
State Key Laboratory of Molecular Reaction Dynamics, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, China; University of Chinese Academy of Sciences, Beijing, 100049, China.
State Key Laboratory of Molecular Reaction Dynamics, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, China.

Therapeutic monoclonal antibodies (mAbs) have become essential biopharmaceuticals for clinical targeted therapies due to their high specificity, affinity and low side effects. The specificity and affinity of mAb to targeting antigen are mainly dependent on the three complementarity determining regions (CDRs) with high variations in amino acid sequences. Therefore, mAb CDR sequencing is crucial for the characterization of therapeutic mAbs. Here, we developed a 193-nm ultraviolet photodissociation (UVPD) based multiple charge integration middle-down mass spectrometry (MCI-MDMS) strategy for mAb sequencing.

中文翻译：

使用多电荷整合、自middle-down策略和紫外光解离对单克隆抗体可变区进行测序

治疗性单克隆抗体（mAb）因其高特异性、亲和力和低副作用而成为临床靶向治疗的重要生物制药。mAb 对靶向抗原的特异性和亲和力主要取决于氨基酸序列高度变化的三个互补决定区（CDR）。因此，mAb CDR 测序对于治疗性 mAb 的表征至关重要。在这里，我们开发了一种基于 193 nm 紫外光解离（UVPD）的多电荷积分自中而下质谱（MCI-MDMS）策略，用于 mAb 测序。

更新日期：2024-11-19

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