Conventional administration of drugs to the inner ear involves therapeutic instability and non-specificity of release, and to overcome these limitations, various drug delivery systems have been developed. The aim of this study was to prepare and characterize peptides-functionalized oligochitosan microcapsules loaded with Dexamethasone and magnetic nanoparticles, which can be used for dual active targeted treatment of inner ear inflammation. The diameter of spherical microcapsules in aqueous solutions was found in the micrometer range. In vitro dexamethasone release kinetics, capsules biodegradation, haemolysis and cellular viability on V79-4 normal cells were also investigated. The release efficiency of dexamethasone from the microcapsules was between 74% and 99.8% after 24 hours. Obtained results indicated that all analyzed microcapsules showed hemolysis degrees lower than 3%, which demonstrated their non-hemolytic character. The viability and morphology tests on V79-4 cells depended on the administered dose and after a 48-hour treatment, all analyzed capsules showed a non-toxic, weak or moderately cytotoxic effect.

中文翻译：

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载有地塞米松的肽功能化磁性微胶囊，用于内耳炎症的双重主动靶向治疗


传统的内耳给药涉及治疗不稳定和非特异性释放，为了克服这些限制，已经开发了各种药物输送系统。本研究的目的是制备和表征载有地塞米松和磁性纳米颗粒的肽功能化寡壳聚糖微胶囊，可用于内耳炎症的双重主动靶向治疗。球形微胶囊在水溶液中的直径在微米范围内。还研究了体外地塞米松释放动力学、胶囊生物降解、溶血和对 V79-4 正常细胞的细胞活力。24 小时后，地塞米松从微胶囊中的释放效率在 74% 至 99.8% 之间。获得的结果表明，所有分析的微胶囊均显示溶血程度低于 3%，这表明其非溶血特性。V79-4 细胞的活力和形态学测试取决于给药剂量，治疗 48 小时后，所有分析的胶囊均显示出无毒、弱或中度细胞毒性作用。