Owing to the hypoglycemic, anti-inflammatory, and other beneficial bioactivities of Moringa oleifera seeds (MOS), the antioxidant activities and physicochemical characteristics of deproteinized polysaccharides extracted using hot water (MSWP) and alkali (MSAP) from MOS were systematically studied. MSAP exhibited higher antioxidant activity than MSWP in vitro. Subsequently, the antioxidant capacity was evaluated after oral administration of MSAP in diabetic rats. The results demonstrated a statistically significant increase in serum antioxidant parameters. The two polysaccharides were then purified and characterized. The molecular weights of purified fractions MSAP3 and MSWP1 were 4.582 × 104 Da and 1.9058 × 105 Da, respectively; the content of uronic acid in MSAP3 was higher than that in MSWP1; and both compounds presented typical infrared spectral absorption peaks of polysaccharides. In conclusion, MSAP exhibits superior antioxidant properties that depend on its structural characteristics, and this study provides data support for further elucidation of the hypoglycemic mechanism of MSAP.

中文翻译：

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辣木种子多糖提取-功能关系在抗氧化能力方面的比较研究


由于辣木种子 （MOS） 具有降血糖、抗炎和其他有益的生物活性，系统研究了使用热水 （MSWP） 和碱 （MSAP） 从 MOS 中提取的脱蛋白多糖的抗氧化活性和理化特性。MSAP 在体外表现出比 MSWP 更高的抗氧化活性。随后，评估糖尿病大鼠口服 MSAP 后的抗氧化能力。结果表明血清抗氧化参数有统计学意义的增加。然后对两种多糖进行纯化和表征。纯化级分 MSAP3 和 MSWP1 的分子量分别为 4.582 × 104 Da 和 1.9058 × 105 Da;MSAP3 中糖醛酸含量高于 MSWP1;两种化合物均呈现多糖的典型红外光谱吸收峰。综上所述，MSAP 表现出优异的抗氧化特性，这取决于其结构特性，本研究为进一步阐明 MSAP 的降糖机制提供了数据支持。