Thiacloprid (THI) is a neonicotinoid insecticide, and its residues pose potential risks to the ecological environment and human health. In this study, computer simulation technology was applied to analyze THI haptens to ensure the similarity in spatial structure, charge properties, and potential distribution. And anti-THI monoclonal antibodies insensitive to matrix effects were prepared using sample extraction solution to simulate the matrix environment, with the half-maximal inhibitory concentration of 0.070 ng/mL in the matrix environment. Furthermore, a colloidal gold immunochromatographic assay (ICA) was established for the detection of THI residues in fruit and vegetable samples, with a quantitative detection range of 0.880–11.091 μg/kg, 1.669–14.056 μg/kg, 31.570–586.121 μg/kg, and 42.291–778.803 μg/kg in grapes, oranges, cucumbers, and tomatoes, respectively. Real sample testing indicated that this method had good consistency with LC-MS/MS. Therefore, the ICA withstood matrix interference, accurately and effectively determined THI residues in fruit and vegetable samples.

中文翻译：

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抗基质干扰的抗噻虫啉单克隆抗体的生产及其在定量免疫层析测定中的应用


噻虫啉 （THI） 是一种新烟碱类杀虫剂，其残留物对生态环境和人类健康构成潜在风险。在本研究中，应用计算机模拟技术分析 THI 半抗原，以确保空间结构、电荷特性和电位分布的相似性。使用样品提取溶液制备对基质效应不敏感的抗THI单克隆抗体，模拟基质环境，基质环境中抑制浓度的一半最大为0.070 ng/mL。此外，建立了胶体金免疫层析法 （ICA） 检测水果和蔬菜样品中的 THI 残留，定量检测范围分别为 0.880–11.091 μg/kg、1.669–14.056 μg/kg、31.570–586.121 μg/kg 和 42.291–778.803 μg/kg葡萄、橙子、黄瓜和西红柿。实样检测表明，该方法与 LC-MS/MS 具有良好的一致性。因此，ICA 能够承受基质干扰，准确有效地测定水果和蔬菜样品中的 THI 残留。