As a major crop pest, common cutworm (CCW) Spodoptera litura (Fabricius) has evolved resistance to several insecticides. Cyproflanilide is a novel meta-diamide insecticide targeting the γ-aminobutyric acid receptor (GABAR), and displays excellent insecticidal activity against lepidopteran pests, including CCW. Therefore, the resistance risk of CCW against cyproflanilide was assessed and the fitness cost was evaluated as well. After exposure with cyproflanilide for 10 generations (F10), the LC50 values of CCW against cyproflanilide increased 1.26 - fold. When the realized heritability (h2) to cyproflanilide in the field was assumed to be the laboratory-estimated value (h2 = 0.09) and the mortality of CCW was 50–90 %, 126–57 generations were required to obtain a 10-fold resistance against cyproflanilide. After selected with cyproflanilide for 10 generations, the egg duration, larval duration, pre-pupal duration and female pupal weight were significantly increased, but the pupal duration, pupation rate and life-cycle rate were significantly decreased. The mean generation time (T) of F10 was significantly prolonged by 4.39 days. The value of relative fitness (Rf) in F10 was 0.99. Although resistance developed slowly, the prolonged egg and larval durations have implications for managing CCW in the field. This information will contribute to identifying practices to delay the resistance development of CCW to cyproflanilide.

中文翻译：

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环丙草酰亚胺在普通切虫斜纹夜蛾（鳞翅目：夜蛾科）中的抗性风险评估及适宜成本


作为一种主要的农作物害虫，普通切虫 （CCW） 斜纹夜蛾 （Fabricius） 已经进化出对几种杀虫剂的耐药性。Cyproflanilide 是一种靶向 γ-氨基丁酸受体 （GABAR） 的新型间二酰胺杀虫剂，对鳞翅目害虫（包括 CCW）表现出优异的杀虫活性。因此，评估了 CCW 对环丙酰腺苷的耐药风险，并评估了适应成本。用环草酰亚胺暴露 10 代 （F10） 后，CCW 对环草酰亚胺的 LC50 值增加了 1.26 倍。当假设现场对环酰苯胺的已实现遗传力 （h2） 为实验室估计值 （h2 = 0.09） 且 CCW 的死亡率为 50-90% 时，需要 126-57 代才能获得对环酰苯胺的 10 倍抗性。用环丙草酰亚胺选择10代后，卵持续时间、幼虫持续时间、前蛹持续时间和雌蛹体重显著增加，但蛹持续时间、化蛹率和生命周期速率显著降低。F10 的平均生成时间 （T） 显著延长了 4.39 d。F10 中的相对适应度 （Rf） 值为 0.99。尽管耐药性发展缓慢，但卵和幼虫持续时间延长对现场管理 CCW 有影响。这些信息将有助于确定延迟 CCW 对环丙酰亚胺耐药发展的做法。