Verticillium wilt caused by Verticillium dahliae is one of the most devastating diseases of cotton worldwide but little is known about resistance of cotton to V. dahliae for phenotypic quantifying. The aim of the current study was to phenotypically characterize resistance of 18 advanced cotton lines to defoliating pathotype (Vd-34) of V. dahliae. Experiments were set up in a greenhouse in two consecutive years. Phenotyping of plant response to V. dahliae was quantified by assessing five agronomic traits (defoliation rate, plant height, root weight, stem diameter, biomass) and internal vascular discoloration. Reaction types were established using area under the disease progress curve (AUDPC). The defoliating pathotype Vd-34 induced significant (P ˂ 0.01) reductions in all the examined agrononic traits. Overall reductions in root weight, defoliation rate, plant height, biomass and stem diameter were detected as 42.33, 34.13, 33.56, 32.31 and 26.63%, respectively. Based on the AUDPC values, of the 18 advanced cotton lines, AntV-17, AntV-19 and AntV-20 were detected as moderately resistant to the defoliating pathotype of V. dahliae, while the others showed moderately susceptible, susceptible and very susceptible reactions. The disease quantifying parameters (AUDPC, rAUDPC and vascular disease score) significantly (P ˂ 0.01) and positively correlated with the reductions in the examined agronomic traits. To our knowledge, this is the first detailed phenotyping for resistance to defoliating pathotype of V. dahliae in cotton and reveals new knowledge to quantify cotton resistance to V. dahliae.

中文翻译：

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高级棉花品系对大丽轮枝菌落叶病型的抗性表型表征


由大丽轮枝菌引起的黄萎病是全世界棉花最具破坏性的疾病之一，但人们对棉花对大丽轮枝菌的抗性知之甚少，用于表型定量。本研究的目的是表型表征 18 种高级棉花品系对大丽轮枝菌落叶病型 （Vd-34） 的抗性。连续两年在温室中进行实验。通过评估 5 种农艺性状 （落叶率、株高、根重、茎直径、生物量） 和内部维管变色，量化植物对大丽轮枝杆菌反应的表型。使用疾病进展曲线下面积 （AUDPC） 确定反应类型。落叶病型 Vd-34 诱导所有检查的 agrononic 性状显着减少 （P ˂ 0.01）。根重、脱叶率、株高、生物量和茎粗的总体降低分别为 42.33 、 34.13 、 33.56 、 32.31 和 26.63%。根据 AUDPC 值，在 18 个高级棉品系中，AntV-17、AntV-19 和 AntV-20 对大丽轮枝菌的落叶病型具有中度抗性，而其他品系则表现出中度敏感、敏感和非常敏感的反应。疾病量化参数 （AUDPC 、 rAUDPC 和血管疾病评分） 显著 （P ˂ 0.01） 并且与所检查的农艺性状的减少呈正相关。据我们所知，这是棉花中对大丽轮枝菌落叶病型抗性的首个详细表型，并揭示了量化棉花对大丽轮枝菌抗性的新知识。