Cucurbitaceous crops are a key group of vegetables widely cultivated in India. Characterized yellowing symptoms along with interveinal chlorosis, mottling and downward curling were observed in ridge gourd, snake gourd, and bottle gourd along with the presence of whiteflies (Bemisia tabaci). To investigate the infecting virus, RT-PCR assays followed by sequencing and Transmission Electron Microscopy (TEM) analysis were performed. Sanger sequencing, nucleotide sequence analysis, and TEM imaging confirmed the association of cucurbit yellow stunting disorder virus (CYSDV), genus Crinivirus within the family Closteroviridae. This study reports the natural occurrence of CYSDV in ridge gourd, snake gourd, and bottle gourd for the first time in India.

中文翻译：

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印度首次报道葫芦黄发育迟缓症病毒导致主要葫芦黄化病


葫芦科作物是印度广泛种植的关键蔬菜组。在葫芦、蛇瓜和葫芦中观察到特征性的黄化症状以及叶脉间萎黄、斑驳和向下卷曲，并伴有粉虱 （Bemisia tabaci） 的存在。为了研究感染病毒，进行了 RT-PCR 检测，然后进行了测序和透射电子显微镜 （TEM） 分析。Sanger 测序、核苷酸序列分析和 TEM 成像证实了葫芦黄发育迟缓疾病病毒 （CYSDV） 的关联，该病毒属是梭状病毒科的 Crinivirus 属。本研究首次在印度报道了 CYSDV 在葫芦、蛇瓜和葫芦中自然存在。