Aphidoletes aphidimyza (Rondani) (Diptera: Cecidomyiidae) is widely used in aphid biological control, and low-temperature storage helps expand its population. Nonetheless, low-temperature effects on A. aphidimyza are unknown. A. aphidimyza pupae were exposed to three cold storage methods lasting 10, 20, and 30 days, respectively: storing at 7 °C for 4 h then 5 °C for 20 h (T1); storing at 9 °C for 4 h then 5 °C for 20 h (T2); and storing at 5 °C for 24 h (T3). The emergence rate under variable low-temperature storage (T1: 81.98%; T2: 81.28%) was significantly higher than that under constant low-temperature storage (T3: 74.98%). Additionally, the emergence rate decreased markedly with prolonged refrigeration time. Egg production at all three refrigeration temperatures declined significantly compared to the control treatment. It is noteworthy that low-temperature storage can significantly prolong the development time of offspring larvae without affecting the overall developmental period of the offspring. After comparing the content of cold-resistant substances in pupae under different low-temperature storage, the total sugar, glycerol, and trehalose increased with an extended storage time. The lifetime feeding capacity of A. aphidimyza offspring larvae on Myzus persicae (Sulzer) improved after cold storage. The functional response curve was aligned with the Holling Ⅱ model. Compared to the control treatment, both T1, T2, and T3 improved the search ability of 3rd-instar larvae in the offspring after 20 and 30 days of refrigeration. Our study indicated that cold storage of A. aphidimyza can extend offspring larval development period and improve its predation ability.

中文翻译：

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Aphidoletes aphidimyza 冷藏可延长子代幼虫发育期，提高其捕食能力


蚜虫 （Rondani） （双翅目：Cecidomyiidae） 广泛用于蚜虫生物防治，低温储存有助于扩大其种群。尽管如此，低温对蚜虫的影响尚不清楚。A. aphidimyza 蛹暴露于三种冷藏方法中，分别持续 10 、 20 和 30 天：在 7 °C 下储存 4 h，然后在 5 °C 下储存 20 h （T1）;在 9 °C 下储存 4 小时，然后在 5 °C 下储存 20 小时 （T2）;并在 5 °C 下储存 24 小时 （T3）。可变低温贮藏下出苗率（T1： 81.98%;T2： 81.28%）显著高于恒定低温贮藏下（T3： 74.98%）。此外，随着冷藏时间的延长，出苗率显着降低。与对照处理相比，所有三种冷藏温度下的产蛋量均显著下降。值得注意的是，低温贮藏可以显著延长子代幼虫的发育时间，而不影响子代的整体发育期。比较不同低温贮藏下蛹中耐寒物质的含量后，总糖、甘油和海藻糖随贮藏时间的延长而增加。冷藏后，A. aphidimyza 后代幼虫对 Myzus persicae （Sulzer） 的终生摄食能力得到改善。功能反应曲线与 Holling II. 模型一致。与对照处理相比，冷藏 20 d 和 30 d 后 T1 、 T2 和 T3 均提高了对子代 3 龄幼虫的搜索能力。研究表明，蚜虫冷藏可以延长后代幼虫发育期并提高其捕食能力。