In situ monitoring of the uptake and transportation process of nano-pesticides during crop growth remains challenging thus far. In this report, the different three sizes of surface-enhanced Raman spectroscopy probes of nano-pesticides loaded with ferbam and acetamiprid are representative non-systemic or systemic pesticides, respectively. The probes were verified to have strong signals of the Raman spectrum enhancement effect. They were further applied on the leaves and roots of tea plants. Within 5 h, the nano-pesticides of 50 nm and 100 nm except 150 nm probes could rapidly penetrate young tea leaves to a depth of about 150–290 μm. Whereas none of the probes were observed to penetrate mature leaves and roots of tea plants. The result showed that the penetration rate depended on the size of the nano-pesticides and the structure or maturity of the plant tissue rather than the loaded pesticides. The penetration rate of small-sized nano-pesticides in young tea leaves is the fastest.

中文翻译：

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使用表面增强拉曼光谱实时原位追踪纳米农药在茶树中的渗透和吸收行为


到目前为止，对作物生长过程中纳米农药的吸收和运输过程进行原位监测仍然具有挑战性。在本报告中，负载 ferbam 和 acetamiprid 的纳米农药的三种不同尺寸的表面增强拉曼光谱探针分别是代表性的非内吸性或内吸性农药。经验证，探针具有较强的拉曼光谱增强效应信号。它们进一步应用于茶树的叶子和根部。在 5 小时内，除 150 nm 探针外，50 nm 和 100 nm 的纳米农药可迅速穿透茶叶幼叶，深度约为 150–290 μm。而没有观察到任何探针穿透茶树的成熟叶子和根。结果表明，渗透率取决于纳米农药的大小和植物组织的结构或成熟度，而不是负载的农药。小尺寸纳米农药在茶叶幼苗中的渗透速度最快。