Flow injection analysis and liquid chromatography are frequently combined with electrochemiluminescence (ECL) for flow analysis. Almost all electrochemistry flow analyses employ traditional three-electrode electrochemical flow cells which have working electrode, counter electrode, and reference electrode; however, it is expensive and difficult to fabricate a traditional three-electrode electrochemical flow cell and inconvenient to renew the electrode. In this study, we have developed a single-electrode flow cell using commercially available conductive polyethylene film as the only electrode through potential differences induced by the electrode resistance for the first time. The single-electrode flow cell features a simple structure, easy renewal of the electrode, and low cost compared to the traditional three-electrode electrochemical flow cells. Taking the typical Ru(bpy)₃²⁺/oxalate ECL system as the analytical model, flow analysis of clinically important oxalate was achieved using single-electrode flow cell. A regression linear equation was obtained over the oxalate concentration ranges from 1 to 200 μM, with a detection limit of 0.92 μM. The single-electrode flow cell is promising for ECL flow analysis.

中文翻译：

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用于电化学发光流动分析的单电极流通池


流式注射分析和液相色谱法经常与电化学发光 （ECL） 相结合进行流式分析。几乎所有的电化学流动分析都采用传统的三电极电化学流通池，这些流通池具有工作电极、对电极和参比电极;然而，传统的三电极电化学流通池制造成本高、难度大，且不方便更新电极。在这项研究中，我们首次通过电极电阻感应的电位差，使用市售的导电聚乙烯薄膜作为唯一的电极，开发了一种单电极流通池。与传统的三电极电化学流通池相比，单电极流通池结构简单，电极易于更新，成本低。以典型的 Ru（bpy）₃²⁺/草酸盐 ECL 系统为分析模型，使用单电极流通池对临床上重要的草酸盐进行流式分析。在 1 至 200 μM 的草酸盐浓度范围内获得回归线性方程，检测限为 0.92 μM。单电极流通池很有前途用于 ECL 流式分析。