β-Galactosidase (β-gal) has emerged as a pivotal biomarker in primary ovarian cancer. Despite the existence of numerous fluorescent probes for β-gal activity detection, quinone methide-based immobilizing probes were shown to avoid rapid diffusion of the activated fluorophore and improve the resolution. However, the synthesis of these fluorophores, particularly near-infrared fluorophores, still exhibits lower efficiency. In this study, we introduce modular and rapidly assembled self-immobilizing fluorogenic probes, capitalizing on the proximity labeling properties of quinone methide (QM). Compared to conventional fluorescent probes, these new probes not only exhibit a fluorogenic response but also achieve permanent retention, demonstrating improved detection sensitivity, particularly after cell fixation and in vivo animal model studies. This straightforward synthesis approach holds promise for broader applications in detecting other analytes.

中文翻译：

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用于 β-半乳糖苷酶检测的自固定荧光探针的模块化和快速组装


β-半乳糖苷酶 （β-gal） 已成为原发性卵巢癌的关键生物标志物。尽管存在许多用于 β-gal 活性检测的荧光探针，但基于醌甲基化物的固定探针被证明可以避免活化荧光团的快速扩散并提高分辨率。然而，这些荧光团，特别是近红外荧光团的合成仍然表现出较低的效率。在这项研究中，我们利用醌甲基化物 （QM） 的邻近标记特性，引入了模块化和快速组装的自固定荧光探针。与传统荧光探针相比，这些新探针不仅表现出荧光反应，而且能够实现永久保留，表现出更高的检测灵敏度，尤其是在细胞固定和体内动物模型研究之后。这种简单的合成方法有望在检测其他分析物方面实现更广泛的应用。