Postoperative therapies for osteosarcoma present substantial challenges due to tumor recurrence and extensive bone defects. To tackle these challenges, laser powder bed fusion is utilized to fabricate biodegradable Zn-Li porous scaffolds that supress tumors and promote osteogenesis. After the structure design and composition selection, the Zn-0.8Li porous scaffold with Gyroid unit optimally balances the co-release of Zn²⁺ and Li⁺ during degradation, resulting in favorable antitumor and osteogenic effects. In vitro, the Zn-0.8Li scaffold significantly inhibits osteosarcoma progression by suppressing tumor cell proliferation, promoting apoptosis, alleviating migration, and simultaneously promotes osteogenic differentiation through the enhanced expression of osteogenic markers. In vivo, the Zn-0.8Li scaffold inhibits the malignant osteosarcoma behavior and facilitates bone regeneration in areas with bone defects. Transcriptomic analysis further reveals that the simultaneous release of Zn²⁺ and Li⁺ from the biodegradable Zn-0.8Li scaffold contributes to anti-osteosarcoma activity by downregulating PI3K/Akt signaling pathways. Taken together, the Zn-0.8Li porous scaffold fabricated using laser powder bed fusion with enhanced antitumor and osteogenic properties is a promising alternative for the postoperative management of osteosarcoma.

中文翻译：

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增材制造的可生物降解 Zn 基多孔支架，以抑制骨肉瘤并促进成骨


由于肿瘤复发和广泛的骨缺损，骨肉瘤的术后治疗面临巨大挑战。为了应对这些挑战，激光粉末床熔融用于制造可生物降解的 Zn-Li 多孔支架，以抑制肿瘤并促进成骨。经过结构设计和成分选择，带有 Gyroid 单元的 Zn-0.8Li 多孔支架在降解过程中最佳平衡了 Zn²⁺ 和 Li⁺ 的共释放，从而具有良好的抗肿瘤和成骨效果。在体外，Zn-0.8Li 支架通过抑制肿瘤细胞增殖、促进细胞凋亡、缓解迁移来显着抑制骨肉瘤进展，同时通过增强成骨标志物的表达促进成骨分化。在体内，Zn-0.8Li 支架抑制恶性骨肉瘤行为并促进骨缺损区域的骨再生。转录组学分析进一步揭示，从可生物降解的 Zn-0.8Li 支架中同时释放 Zn²⁺ 和 Li⁺ 通过下调 PI3K/Akt 信号通路有助于抗骨肉瘤活性。综上所述，使用激光粉末床熔融制备的 Zn-0.8Li 多孔支架具有增强的抗肿瘤和成骨性能，是骨肉瘤术后治疗的有前途的替代方案。