The impact of carbon/nitrogen (C/N) ratio on sequencing batch biofilm reactor (SBBR) initiated with different seed sludges for treating actual mariculture effluent was explored. Increasing the C/N ratio significantly enhanced the nitrogen removal efficiency, achieving average removal efficiency of 95% for ammonia nitrogen and 73% for total nitrogen at ratio of 30, while the impact of seed sludge was minimal. High C/N ratio promoted the secretion of tightly bound extracellular polymeric substances (TB-EPS), which showed significant correlation with nitrogen removal. Interactions between bacteria and archaea were enhanced and conditionally rare or abundant taxa were the keystone taxa. High C/N ratio inhibited the relative abundance of ammonia-oxidizing archaea (Candidatus_Nitrosopumilus) and bacteria (Nitrosomonas), but promoted the heterotrophic nitrification-aerobic denitrification bacteria (Halomonas). The expression of nitrogen removal functional genes significantly correlated with functional genera. This study emphasized the crucial role of high C/N ratios in biological nitrogen removal from actual mariculture effluent.

中文翻译：

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碳/氮比对用不同种子污泥启动的序批生物膜反应器的影响，以处理实际海水养殖废水


探讨了碳/氮 （C/N） 比值对用不同种子污泥启动的测序分批生物膜反应器 （SBBR） 处理实际海水养殖废水的影响。提高 C/N 比显著提高了脱氮效率，在 30 比下，氨氮的平均脱氮效率为 95%，总氮的平均脱氮效率为 73%，而种子污泥的影响很小。高 C/N 比促进了紧密结合的细胞外聚合物物质 （TB-EPS） 的分泌，这与脱氮呈显著相关性。细菌和古细菌之间的相互作用得到增强，有条件的稀有或丰富的分类群是关键分类群。高 C/N 比值抑制氨氧化古细菌 （Candidatus_Nitrosopumilus） 和细菌 （Nitrosomonas） 的相对丰度，但促进异养硝化-需氧反硝化细菌 （Halomonas）。脱氮功能基因的表达与功能属显著相关。本研究强调了高 C/N 比在从实际海水养殖污水中去除生物氮中的关键作用。