Although prokaryotic microbes in coking wastewater (CWW) treatment have been comprehensively studied, the ecological functions of viruses remain unclear. A full-scale CWW biological treatment AOHO combination was studied for the virus-bacterium interactions involved in element cycles by metaviromics, metagenomics and physicochemical characteristics. Results showed the unique viromic profile with Cirlivirales and Petitvirales as the dominant viruses infecting functional bacteria hosts. The auxiliary metabolic genes (AMGs) focused on element cycles, including metabolisms of carbon (fadA), nitrogen (glnA), sulfur (mddA and cysK) and phosphorus (phoH). Other AMGs were involved in toxic tolerance of hosts, improving their cell membrane and wall robustness, antioxidant, DNA repair and cobalamin biosynthesis. Vice versa, the bloomed host provided fitness advantages for viruses. Dissolved oxygen was found to be the key factor shaping the distributions of viral community and AMGs. Summarizing, the study exposed the mutual virus-bacterium interaction in the AOHO combination providing stable treatment efficiency.

中文翻译：

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焦化废水生物处理中元素循环中涉及的病毒-细菌相互作用


尽管已经对焦化废水 （CWW） 处理中的原核微生物进行了全面研究，但病毒的生态功能仍不清楚。通过宏病毒组学、宏基因组学和理化特性研究了全尺寸 CWW 生物处理 AOHO 组合对元素循环中涉及的病毒-细菌相互作用。结果显示，以 Cirlivirales 和 Petitvirales 为主要病毒感染功能性细菌宿主，具有独特的病毒特征。辅助代谢基因 （AMGs） 专注于元素循环，包括碳 （fadA） 、氮 （glnA） 、硫 （mddA 和 cysK） 和磷 （phoH） 的代谢。其他 AMGs 参与宿主的毒性耐受性，提高其细胞膜和细胞壁的稳健性、抗氧化、DNA 修复和钴胺素生物合成。反之亦然，开花的宿主为病毒提供了健身优势。发现溶解氧是影响病毒群落和 AMG 分布的关键因素。综上所述，该研究揭示了 AOHO 组合中病毒-细菌的相互作用，提供了稳定的治疗效果。