The reliable stimulation and recording of electrical activity in single cells by means of organic bio‐electronics will be an important milestone in developing new low‐cost and highly biocompatible medical devices. This paper demonstrates extracellular voltage stimulation and single‐cell membrane potential recording by means of a dual‐gate electrolyte‐gated organic field‐effect transistors (EGOFET) employing 2,8‐Difluoro‐5,11‐bis(triethylsilylethynyl)anthradithiophene blended with polystyrene as active material. To obtain a sufficiently small footprint to allow bidirectional communication at the single cell level, the EGOFET technology has been scaled down implementing a Corbino layout, paving the way to the development of novel bidirectional Electrocorticography (ECoG) devices with a high spatial resolution. A specific and thorough analysis of the working mechanisms of EGOFET‐based bio‐sensors is reported, highlighting the importance of the device design and using an appropriate batch of measurements for the recording of the electrical activity of cells.

中文翻译：

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通过电解质门控有机场效应晶体管进行单细胞膜电位刺激和记录


通过有机生物电子学对单细胞中的电活动进行可靠的刺激和记录将是开发新的低成本和高度生物相容性医疗设备的重要里程碑。本文通过采用 2,8-二氟-5,11-双（三乙基甲硅烷基乙炔基）蒽噻吩与聚苯乙烯共混作为活性材料，通过双门电解质门控有机场效应晶体管 （EGOFET） 展示了细胞外电压刺激和单细胞膜电位记录。为了获得足够小的占用空间以允许单单元级别的双向通信，EGOFET 技术已按比例缩小，实现了 Corbino 布局，为开发具有高空间分辨率的新型双向皮层电图 （ECoG） 设备铺平了道路。报告了对基于 EGOFET 的生物传感器的工作机制的具体而彻底的分析，强调了设备设计的重要性，并使用适当的测量批次来记录细胞的电活动。