Purpose

Accelerated grassland degradation due to global climate change and intensified human activities necessitates green and sustainable ecological restoration measures. Microbial and organic fertilizer composites have shown positive effects in agricultural soil improvement. However, the effects on soil nutrient cycling and plant growth of microbial inoculants alone or in combination with organic fertilizers on the multifunctionality of natural ecosystems remain unclear.

Methods

This study explores the effects of Bacillus subtilis, Azotobacter salinestris, and Claroideoglomus lamellosum (added alone or in all possible combinations), applied in conjunction with organic fertilizers at different inoculation levels on the physicochemical properties of degraded soil, microbial factors, and Leymus chinensis plant biomass through pot experiments.

Results

Results indicate that combining organic fertilizers with B. subtilis, A. salinestris, and C. lamellosum enhances soil nutrient availability. Compared to single organic fertilizer treatments, all mixed inoculation schemes increased nutrient uptake. The combined inoculation of A. salinestris and C. lamellosum is most effective, resulting in a remarkable 465% increase in belowground biomass and significantly enhancing soil nutrient content, particularly with increases of 247% in NH + 4-N and 348% in NO- 3-N. Soil enrichment with external nutrients can influence the strength of their interactions with different factors.

Conclusions

The study demonstrates that co-inoculation of bacteria and fungi has a synergistic effect on increasing plant biomass and soil nutrient availability. This provides essential ecological theories and practical scientific evidence for using microorganisms to improve the ecological functions of degraded grasslands, maintain soil health, and ensure the sustainable development of grasslands.

中文翻译：

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固氮菌与菌根真菌结合促进羊草生长和半干旱区退化草原的生物修复

 目的

由于全球气候变化和人类活动的加剧，草原退化加速，需要采取绿色和可持续的生态恢复措施。微生物和有机肥料复合材料在农业土壤改良方面显示出积极作用。然而，单独使用微生物接种剂或与有机肥料结合使用对土壤养分循环和植物生长对自然生态系统多功能性的影响仍不清楚。

 方法

本研究通过盆栽试验，探讨了枯草芽孢杆菌、盐水固氮杆菌和Claroideoglomus lamellosum（单独添加或以所有可能的组合）与有机肥配施对退化土壤理化性质、微生物因子和羊草植物生物量的影响。

 结果

结果表明，将有机肥料与枯草芽孢杆菌、盐渍芽孢杆菌和板状芽孢杆菌混合使用可以提高土壤养分可用性。与单一有机肥处理相比，所有混合接种方案都增加了养分吸收。盐水蚤和蚜蒲的联合接种最有效，使地下生物量显著增加 465%，并显著提高土壤养分含量，特别是 NH + 4-N 增加 247%，NO-3-N 增加 348%。

 结论

研究表明，细菌和真菌的共接种对增加植物生物量和土壤养分可用性具有协同作用。这为利用微生物改善退化草原的生态功能，维护土壤健康，保障草原的可持续发展提供了必要的生态理论和实际科学证据。