A ligand-responsive artificial protein–protein communication (LIRAC) was developed by redefining the connection between transcription factors (TFs) and CRISPR/Cas enzymes that do not have natural interactions. LIRAC immediately transduces target-induced TF allostery into fast activation of CRISPR/Cas enzymes within a homogeneous system, allowing for rapid response, tunable dynamic range, tristate logic, and portable detection.

中文翻译：

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配体响应性人工蛋白质-蛋白质通讯，用于场部署的无细胞生物传感


通过重新定义转录因子 （TFs） 和没有自然相互作用的 CRISPR/Cas 酶之间的联系，开发了一种配体响应性人工蛋白质-蛋白质通讯 （LIRAC）。LIRAC 立即将靶标诱导的 TF 变构转导为均相系统内 CRISPR/Cas 酶的快速激活，从而实现快速响应、可调动态范围、三态逻辑和便携式检测。