High energy diets are a risk factor for intestinal barrier damage. Butyrate, a major energy source for intestinal epithelial cells, has been shown to improve barrier dysfunction and modulate the gut microbiota. In this trial, we examined the preventative effect of coated sodium butyrate (CSB) on high-energy and low-protein (HELP)-induced intestinal barrier injury in laying hens, and also worked to determine the underlying mechanisms by an integrative analysis of gut microbiota and the metabolome. A total of 216 healthy 28-week-old Huafeng laying hens were randomly assigned to 3 groups with 6 replicates each: the CON group (normal diet), HELP group (HELP diet) and CH500 group (500 mg/kg CSB added to HELP diet). The duration of the trial encompassed a period of 10 weeks. The results revealed that CSB treatment improved the laying rate and mitigated the detrimental effects on intestinal barrier function and the inflammatory response induced by the HELP diet in laying hens (P < 0.05). Microbial profiling analysis revealed that the CSB treatment reshaped the HELP-perturbed gut microbiota and promoted the growth of beneficial bacteria (P < 0.05). Untargeted metabolomics analysis revealed that CSB reduced the metabolites associated with intestinal inflammation (P < 0.05). In conclusion, CSB did not merely modulate alterations in the gut microbiota composition and microbial metabolites but also yielded increased egg production, while mitigating intestinal barrier dysfunction and inflammatory responses induced by HELP in laying hens.

中文翻译：

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用包衣丁酸钠靶向肠道菌群和代谢谱，以改善日粮诱导的蛋鸡高能量和低蛋白肠道屏障功能障碍


高能量饮食是肠道屏障受损的危险因素。丁酸盐是肠上皮细胞的主要能量来源，已被证明可以改善屏障功能障碍并调节肠道微生物群。在这项试验中，我们检查了包衣丁酸钠 （CSB） 对高能量和低蛋白 （HELP） 诱导的蛋鸡肠道屏障损伤的预防作用，并通过肠道菌群和代谢组的综合分析来确定潜在机制。将 216 只健康的 28 周龄华丰蛋鸡随机分为 3 组，每组 6 个重复：CON 组（正常日粮）、HELP 组（HELP 日粮）和 CH500 组（HELP 日粮中添加 500 mg/kg CSB）。试验持续时间为 10 周。结果显示，CSB 处理提高了蛋鸡的产蛋率，减轻了 HELP 饮食对蛋鸡肠道屏障功能和炎症反应的不利影响 （P < 0.05）。微生物分析显示，CSB 处理重塑了受 HELP 扰动的肠道菌群，促进了有益菌的生长 （P < 0.05）。非靶向代谢组学分析显示，CSB 降低了与肠道炎症相关的代谢物 （P < 0.05）。总之，CSB 不仅调节肠道菌群组成和微生物代谢物的改变，而且还增加了产蛋量，同时减轻了 HELP 诱导的蛋鸡肠道屏障功能障碍和炎症反应。