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Sheep-derived butyrate-producing Clostridium beijerinckii R8 alleviates diarrhea by shaping the gut microbiota of goat kids
Animal Nutrition ( IF 6.1 ) Pub Date : 2024-07-14 , DOI: 10.1016/j.aninu.2024.06.004
Dingkun Fan, Yuze Fu, Jixian Zhang, Yanliang Bi, Tao Ma, Qiyu Diao, Naifeng Zhang

Newborn goat kids exposed to environmental stress are susceptible to diarrhea due to immature intestinal functions and undeveloped gut microbiota. Butyrate-producing bacteria as next generation probiotics benefit the maintenance of intestinal health, but the mode of regulation is still unclear. Herein, a novel butyrate-producing strain was isolated from sheep rumen and identified as Clostridium beijerinckii (C. beijerinckii) R8, thereafter goat kids were treated with C. beijerinckii R8 to elucidate its regulatory mechanisms on diarrhea. Thirty-six goat kids were assigned to four groups: control (CON), low dose (LCB; supplementation with 5 × 107 CFU/mL of C. beijerinckii R8, each with 10 mL/d), middle dose (MCB; supplementation with 5 × 108 CFU/mL of C. beijerinckii R8, each with 10 mL/d), high dose (HCB; supplementation with 5 × 109 CFU/mL of C. beijerinckii R8, each with 10 mL/d). The experiment lasted for 15 d, and 6 goat kids were randomly selected from each group for slaughter on the last day of the trial. The results showed that this isolate reduced the diarrhea rate (P < 0.001) and fecal scores (P < 0.001). In the gut, its supplementation inhibited inflammation, increased antioxidant capacity, and regulated intestinal flora. Meanwhile, C. beijerinckii R8 strengthened the intestinal barrier and altered the jejunum morphology. This evidence suggests that C. beijerinckii R8 may alleviate diarrhea in goat kids by regulating microbiota, which is directly related to intestinal barrier and immune capacity.

中文翻译:


绵羊来源的丁酸盐生产梭菌 Clostridium beijerinckii R8 通过塑造山羊幼崽的肠道微生物群来缓解腹泻



由于肠道功能不成熟和肠道微生物群未发育,暴露于环境压力下的新生山羊幼崽容易发生腹泻。产生丁酸盐的细菌作为下一代益生菌,有利于维持肠道健康,但调节模式仍不清楚。本文从绵羊瘤胃中分离出一种新型产丁酸盐菌株,鉴定为北杰林基梭菌 (C. beijerinckii) R8,此后用北杰林基梭 菌 R8 处理山羊幼崽,阐明其对腹泻的调控机制。将 36 只山羊幼崽分为四组:对照组 (CON)、低剂量 (LCB;补充 5 × 107 CFU/mL 北汉金球菌 R8,每组 10 mL/d)、中剂量(MCB;补充 5 × 108 CFU/mL 北金球 菌 R8,每组 10 mL/d)、高剂量(HCB;补充 5 × 109 CFU/mL 北金球菌 R8, 每个 10 mL/d)。实验持续 15 d,每组随机选择 6 只山羊幼崽在试验的最后一天屠宰。结果表明,该分离株降低了腹泻率 (P < 0.001) 和粪便评分 (P < 0.001)。在肠道中,它的补充抑制了炎症,增加了抗氧化能力,并调节了肠道菌群。同时,C. beijerinckii R8 加强了肠道屏障并改变了空肠形态。这一证据表明,C. beijerinckii R8 可能通过调节微生物群来缓解山羊幼崽的腹泻,这与肠道屏障和免疫能力直接相关。
更新日期:2024-07-14
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