This pioneering study is the first to explore the extraction of Calycolpus goetheanus using supercritical carbon dioxide (scCO2), novel approach to harnessing the bioactive potential of this Amazonian species. By examining extractions under varying temperatures (35 and 45 °C) and pressures (150, 250, and 350 bar), the research not only evaluates the yields but also uncovers significant differences in chemical composition and antioxidant activity compared to traditional hydrodistillation. Notably, the highest yield (2.11 ± 0.07 %) was achieved under 45 °C and 350 bar conditions, where n-heneicosane (34.26 %) was the dominant compound, alongside δ-cadinene, α-terpineol, and other important terpenes and fatty alcohols. The scCO2 extracts demonstrated superior antioxidant activity compared to hydrodistillation, highlighting the method's ability to preserve and concentrate bioactive compounds. This research advances the field by showcasing scCO2 as a greener and more efficient extraction technique, with potential applications in pharmaceuticals, cosmetics, and nutraceuticals, opening new pathways for the sustainable exploitation of Amazonian biodiversity.

中文翻译：

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测定超临界 CO2 获得的 Calycolpus goetheanus （O. Berg） 提取物的工艺参数、化学成分和抗氧化活性


这项开创性研究首次探索了使用超临界二氧化碳 （scCO2） 提取 Calycolpus goetheanus，这是利用这种亚马逊物种生物活性潜力的新方法。通过检查不同温度（35 °C 和 45 °C）和压力（150、250 和 350 bar）下的萃取物，该研究不仅评估了产量，还发现了与传统加氢蒸馏相比，化学成分和抗氧化活性的显著差异。值得注意的是，在 45 °C 和 350 bar 条件下实现了最高的收率 （2.11 ± 0.07 %），其中正二十烷 （34.26 %） 是主要化合物，还有 δ-卡丁烯、α-松油醇和其他重要的萜烯和脂肪醇。与加氢蒸馏相比，scCO2 提取物表现出优异的抗氧化活性，突出了该方法保存和浓缩生物活性化合物的能力。这项研究通过将 scCO2 展示为一种更环保、更高效的提取技术来推动该领域的发展，在制药、化妆品和营养保健品方面具有潜在应用，为可持续开发亚马逊生物多样性开辟了新的途径。