Numerous sources of putative novel resistance genes toward Phytophthora sojae (Rps genes) have been identified and loci mapped in soybean (Glycine max L. Merr.) but cloning has remained elusive. We utilized resistance gene enrichment sequencing (RenSeq) to identify the putative resistance genes in 20 plant introductions (PIs) and differentials of the cultivar Williams with rps, Rps1c, Rps3a, and Rps8. The DNA from these genotypes was enriched and sequenced using more than 25,000 80 nt baits designed to nucleotide‐binding leucine‐rich repeat (NLR) encoding sequences. Overall, there were greater numbers of variants in the NLR‐encoding genes in Rps loci on chromosomes (Chrs) 3, 7, 13, and 18 for the 20 PIs as compared to the Williams differentials for rps, Rps1c, Rps1k, Rps3a, and Rps8. Genes encoding Rps1c, Rps3a, and Rps8 were proposed based on sequence differences among the differentials. Among the 20 PIs, there may be additional alleles on Chrs 3, 13, and 18, and PI399079 may have two new alleles at Chrs 3 and 7 loci. A unique NLR on Chr 8 was identified in PI200553. New alleles were also identified on Chrs 3 and 18 when the PI and resistant bulks were compared to susceptible recombinant inbred lines. This study demonstrates the utility of RenSeq as an efficient method to identify and predict specific novel NLR genes in landrace soybean germplasm, which confer resistance to P. sojae and obtain gene‐specific markers to facilitate their introgression into modern cultivars.

中文翻译：

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使用推定的新型和已知 Rps 基因对选定大豆植物引入和差异中大豆疫霉 NLR 基因的抗性基因富集测序


已经确定了许多推定的针对大豆疫霉的新型抗性基因 （Rps 基因） 的来源，并在大豆 （Glycine max L. Merr.） 中定位了位点，但克隆仍然难以捉摸。我们利用抗性基因富集测序 （RenSeq） 来鉴定 20 种植物引入 （PI） 和品种 Williams 的 rps、Rps1c、Rps3a 和 Rps8 差异中的推定抗性基因。来自这些基因型的 DNA 使用超过 25,000 个 80 nt 诱饵进行富集和测序，这些诱饵旨在核苷酸结合富含亮氨酸重复序列 （NLR） 编码序列。总体而言，与 rps、Rps1c、Rps1k、Rps3a 和 Rps8 的 Williams 差异相比，20 个 PIs 上 Rps 基因座中 NLR 编码基因的变异数量更多。编码 Rps1c 、 Rps3a 和 Rps8 的基因是根据差异之间的序列差异提出的。在这 20 个 PI 中，可能在 Chrs 3、13 和 18 上有额外的等位基因，PI399079可能在 Chrs 3 和 7 位点有两个新的等位基因。在 PI200553 年鉴定出 Chr 8 上的唯一 NLR。当将 PI 和抗性块体与易感重组自交系进行比较时，也在 Chrs 3 和 18 上鉴定出新的等位基因。本研究证明了 RenSeq 作为识别和预测地方品种大豆种质中特定新 NLR 基因的有效方法的效用，这些基因赋予大豆 P. sojae 抗性并获得基因特异性标记，以促进它们渗入现代栽培品种。