Proteins are important therapeutic agents, yet better methods are needed to deliver them inside of cells. Polymeric nanocarriers (PNCs) are versatile materials for this purpose, and to enhance their development, it is necessary to quantify protein delivery efficiency into cells by numerous PNC designs at the same time. Current strategies for screening PNC systems are qualitative and mostly serial. Here, we describe a multiplexed approach that uses metal-coded mass tags (MMTs) to quantify the delivery of protein into cells by several different PNC designs simultaneously. Our approach will facilitate the development of more potent delivery systems by improving precision and reducing costs, effort, and time.

中文翻译：

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聚合物纳米载体递送的蛋白质细胞摄取的多重定量筛选


蛋白质是重要的治疗剂，但需要更好的方法将它们输送到细胞内。聚合物纳米载体 （PNC） 是用于此目的的多功能材料，为了促进其开发，有必要同时通过多种 PNC 设计量化蛋白质向细胞中的递送效率。目前筛选 PNC 系统的策略是定性的，并且大多是连续的。在这里，我们描述了一种多重方法，该方法使用金属编码质量标签 （MMT） 来量化几种不同的 PNC 设计同时将蛋白质递送到细胞中。我们的方法将通过提高精度并降低成本、工作量和时间来促进更有效的递送系统的开发。